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Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages

Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages

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SCHILDKNECHT, Stefan, Kathrin HEINZ, Andreas DAIBER, Jürg HAMACHER, Cengiz KAVAKLÍ, Volker ULLRICH, Markus BACHSCHMID, 2006. Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages. In: Biochemical and Biophysical Research Communications. 340(1), pp. 318-325. ISSN 0006-291X. eISSN 1090-2104

@article{Schildknecht2006-02-03Autoc-38920, title={Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages}, year={2006}, doi={10.1016/j.bbrc.2005.12.009}, number={1}, volume={340}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={318--325}, author={Schildknecht, Stefan and Heinz, Kathrin and Daiber, Andreas and Hamacher, Jürg and Kavaklí, Cengiz and Ullrich, Volker and Bachschmid, Markus} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38920"> <dcterms:title>Autocatalytic tyrosine nitration of prostaglandin endoperoxide synthase-2 in LPS-stimulated RAW 264.7 macrophages</dcterms:title> <dc:creator>Bachschmid, Markus</dc:creator> <dc:contributor>Hamacher, Jürg</dc:contributor> <dc:contributor>Daiber, Andreas</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38920"/> <dc:creator>Daiber, Andreas</dc:creator> <dc:creator>Kavaklí, Cengiz</dc:creator> <dc:contributor>Heinz, Kathrin</dc:contributor> <dc:creator>Hamacher, Jürg</dc:creator> <dc:creator>Heinz, Kathrin</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dcterms:available> <dc:creator>Schildknecht, Stefan</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-16T12:39:42Z</dc:date> <dcterms:issued>2006-02-03</dcterms:issued> <dc:contributor>Bachschmid, Markus</dc:contributor> <dc:contributor>Schildknecht, Stefan</dc:contributor> <dcterms:abstract xml:lang="eng">In the literature, biological tyrosine nitrations have been reported to depend not only on peroxynitrite but also on nitrite/hydrogen peroxide linked to catalysis by myeloperoxidase. In endotoxin-stimulated RAW 264.7 macrophages, we have detected a major nitrotyrosine positive protein band around 72 kDa and identified it as prostaglandin endoperoxide synthase-2 (PGHS-2). Isolated PGHS-2 in absence of its substrate arachidonate was not only tyrosine-nitrated with peroxynitrite, but also with nitrite/hydrogen peroxide in complete absence of myeloperoxidase. Our data favor an autocatalytic activation of nitrite by PGHS-2 with a subsequent nitration of the essential tyrosine residue in the cyclooxygenase domain. Under inflammatory conditions, nitrite formed via NO-synthase-2 may therefore act as an endogenous regulator for PGHS-2 in stimulated macrophages. Nitration of PGHS-2 by the autocatalytic activation of nitrite further depends on the intracellular concentration of arachidonate since arachidonate reacted competitively with nitrite and could prevent PGHS-2 from nitration when excessively present.</dcterms:abstract> <dc:creator>Ullrich, Volker</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Kavaklí, Cengiz</dc:contributor> <dc:contributor>Ullrich, Volker</dc:contributor> </rdf:Description> </rdf:RDF>

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