The SAF-box domain of chromatin protein DEK

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BÖHM, Friederike, Ferdinand KAPPES, Ingo SCHOLTEN, Nicole RICHTER, Hiroshi MATSUO, Rolf KNIPPERS, Tanja WALDMANN, 2005. The SAF-box domain of chromatin protein DEK. In: Nucleic Acids Research. 33(3), pp. 1101-1110. ISSN 0301-5610. eISSN 1362-4962

@article{Bohm2005SAF-b-38770, title={The SAF-box domain of chromatin protein DEK}, year={2005}, doi={10.1093/nar/gki258}, number={3}, volume={33}, issn={0301-5610}, journal={Nucleic Acids Research}, pages={1101--1110}, author={Böhm, Friederike and Kappes, Ferdinand and Scholten, Ingo and Richter, Nicole and Matsuo, Hiroshi and Knippers, Rolf and Waldmann, Tanja} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38770"> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38770"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-08T08:43:33Z</dc:date> <dc:creator>Richter, Nicole</dc:creator> <dc:creator>Knippers, Rolf</dc:creator> <dc:contributor>Matsuo, Hiroshi</dc:contributor> <dc:contributor>Kappes, Ferdinand</dc:contributor> <dc:contributor>Richter, Nicole</dc:contributor> <dcterms:abstract xml:lang="eng">DEK is an abundant chromatin protein in metazoans reaching copy numbers of several millions/nucleus. Previous work has shown that human DEK, a protein of 375 amino acids, has two functional DNA-binding domains, of which one resides in a central part of the molecule and contains sequences corresponding to the scaffold attachment factor-box (SAF-box) domain as found in a growing number of nuclear proteins. Isolated SAF-box peptides (amino acids 137-187) bind weakly to DNA in solution, but when many SAF-box peptides are brought into close proximity on the surface of Sephadex beads, cooperative effects lead to a high affinity to DNA. Furthermore, a peptide (amino acids 87-187) that includes a sequence on the N-terminal side of the SAF-box binds efficiently to DNA. This peptide prefers four-way junction DNA over straight DNA and induces supercoils in relaxed circular DNA just like the full-length DEK. Interestingly, however, the 87-187 amino acid peptide introduces negative supercoils in contrast to the full-length DEK, which is known to introduce positive supercoils. We found that two adjacent regions (amino acids 68-87 and 187-250) are necessary for the formation of positive supercoils. Our data contribute to the ongoing characterization of the abundant and ubiquitous DEK chromatin protein.</dcterms:abstract> <dc:contributor>Scholten, Ingo</dc:contributor> <dcterms:issued>2005</dcterms:issued> <dc:creator>Kappes, Ferdinand</dc:creator> <dc:creator>Waldmann, Tanja</dc:creator> <dc:creator>Scholten, Ingo</dc:creator> <dcterms:title>The SAF-box domain of chromatin protein DEK</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-08T08:43:33Z</dcterms:available> <dc:contributor>Knippers, Rolf</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:language>eng</dc:language> <dc:creator>Matsuo, Hiroshi</dc:creator> <dc:creator>Böhm, Friederike</dc:creator> <dc:contributor>Böhm, Friederike</dc:contributor> <dc:contributor>Waldmann, Tanja</dc:contributor> </rdf:Description> </rdf:RDF>

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