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Dynamic <sup>13</sup>C/<sup>1</sup>H NMR imaging uncovers sugar allocation in the living seed : Sucrose allocation in seed

Dynamic 13C/1H NMR imaging uncovers sugar allocation in the living seed : Sucrose allocation in seed

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Prüfsumme: MD5:9ad232c3b8ef75a0286329c1d5dd748f

MELKUS, Gerd, Hardy ROLLETSCHEK, Johannes FUCHS, Volodymyr RADCHUK, Eva GRAFAHREND-BELAU, Nese SREENIVASULU, Twan RUTTEN, Diana WEIER, Falk SCHREIBER, Ljudmilla BORISJUK, 2011. Dynamic 13C/1H NMR imaging uncovers sugar allocation in the living seed : Sucrose allocation in seed. In: Plant Biotechnology Journal. 9(9), pp. 1022-1037. ISSN 1467-7644. eISSN 1467-7652

@article{Melkus2011Dynam-38728, title={Dynamic 13C/1H NMR imaging uncovers sugar allocation in the living seed : Sucrose allocation in seed}, year={2011}, doi={10.1111/j.1467-7652.2011.00618.x}, number={9}, volume={9}, issn={1467-7644}, journal={Plant Biotechnology Journal}, pages={1022--1037}, author={Melkus, Gerd and Rolletschek, Hardy and Fuchs, Johannes and Radchuk, Volodymyr and Grafahrend-Belau, Eva and Sreenivasulu, Nese and Rutten, Twan and Weier, Diana and Schreiber, Falk and Borisjuk, Ljudmilla} }

Radchuk, Volodymyr Fuchs, Johannes Fuchs, Johannes Dynamic <sup>13</sup>C/<sup>1</sup>H NMR imaging uncovers sugar allocation in the living seed : Sucrose allocation in seed Schreiber, Falk Schreiber, Falk Radchuk, Volodymyr Melkus, Gerd Weier, Diana Melkus, Gerd Sreenivasulu, Nese Weier, Diana Grafahrend-Belau, Eva Sreenivasulu, Nese Borisjuk, Ljudmilla Grafahrend-Belau, Eva eng 2017-05-04T09:01:14Z Seed growth and accumulation of storage products relies on the delivery of sucrose from the maternal to the filial tissues. The transport route is hidden inside the seed and has never been visualized in vivo. Our approach, based on high-field nuclear magnetic resonance and a custom made (13)C/(1) H double resonant coil, allows the non-invasive imaging and monitoring of sucrose allocation within the seed. The new technique visualizes the main stream of sucrose and determines its velocity during the grain filling in barley (Hordeum vulgare L.). Quantifiable dynamic images are provided, which allow observing movement of (13)C-sucrose at a sub-millimetre level of resolution. The analysis of genetically modified barley grains (Jekyll transgenic lines, seg8 and Risø13 mutants) demonstrated that sucrose release via the nucellar projection towards the endosperm provides an essential mean for the control of seed growth by maternal organism. The sucrose allocation was further determined by structural and metabolic features of endosperm. Sucrose monitoring was integrated with an in silico flux balance analysis, representing a powerful platform for non-invasive study of seed filling in crops. Rutten, Twan Rolletschek, Hardy 2011 Rolletschek, Hardy 2017-05-04T09:01:14Z Borisjuk, Ljudmilla Rutten, Twan

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