KOPS - The Institutional Repository of the University of Konstanz

X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility

X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

DOGAN, Taner, Gregory S. HARMS, Mirko HEKMAN, Christiaan KARREMAN, Tripat Kaur OBEROI, Emad S. ALNEMRI, Ulf R. RAPP, Krishnaraj RAJALINGAM, 2008. X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility. In: Nature cell biology. 10(12), pp. 1447-1455. ISSN 1465-7392. eISSN 1476-4679. Available under: doi: 10.1038/ncb1804

@article{Dogan2008-12Xlink-38710, title={X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility}, year={2008}, doi={10.1038/ncb1804}, number={12}, volume={10}, issn={1465-7392}, journal={Nature cell biology}, pages={1447--1455}, author={Dogan, Taner and Harms, Gregory S. and Hekman, Mirko and Karreman, Christiaan and Oberoi, Tripat Kaur and Alnemri, Emad S. and Rapp, Ulf R. and Rajalingam, Krishnaraj}, note={There is an Erratum (January 2009) associated with this Article : Nature Cell Biology 11, 110 (2009), http://dx.doi.org/10.1038/ncb0109-110} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38710"> <dc:creator>Hekman, Mirko</dc:creator> <dc:contributor>Oberoi, Tripat Kaur</dc:contributor> <dc:contributor>Alnemri, Emad S.</dc:contributor> <dc:contributor>Harms, Gregory S.</dc:contributor> <dcterms:abstract xml:lang="eng">Inhibitor of apoptosis proteins (IAP) are evolutionarily conserved anti-apoptotic regulators. C-RAF protein kinase is a direct RAS effector protein, which initiates the classical mitogen-activated protein kinase (MAPK) cascade. This signalling cascade mediates diverse biological functions, such as cell growth, proliferation, migration, differentiation and survival. Here we demonstrate that XIAP and c-IAPs bind directly to C-RAF kinase and that siRNA-mediated silencing of XIAP and c-IAPs leads to stabilization of C-RAF in human cells. XIAP binds strongly to C-RAF and promotes the ubiquitylation of C-RAF in vivo through the Hsp90-mediated quality control system, independently of its E3 ligase activity. In addition, XIAP or c-IAP-1/2 knockdown cells showed enhanced cell migration in a C-RAF-dependent manner. XIAP promotes binding of CHIP (carboxy terminal Hsc70-interacting protein), a chaperone-associated ubiquitin ligase, to the C-RAF-Hsp90 complex in vivo. Interfering with CHIP expression resulted in stabilization of C-RAF and enhanced cell migration, as observed in XIAP knockdown cells. Our data show an unexpected role of XIAP and c-IAPs in the turnover of C-RAF protein, thereby modulating the MAPK signalling pathway and cell migration.</dcterms:abstract> <dc:contributor>Hekman, Mirko</dc:contributor> <dc:creator>Karreman, Christiaan</dc:creator> <dc:creator>Alnemri, Emad S.</dc:creator> <dc:contributor>Dogan, Taner</dc:contributor> <dc:contributor>Rajalingam, Krishnaraj</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-04T07:30:05Z</dcterms:available> <dcterms:issued>2008-12</dcterms:issued> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Rajalingam, Krishnaraj</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility</dcterms:title> <dc:creator>Oberoi, Tripat Kaur</dc:creator> <dc:contributor>Karreman, Christiaan</dc:contributor> <dc:creator>Harms, Gregory S.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-04T07:30:05Z</dc:date> <dc:creator>Dogan, Taner</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38710"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Rapp, Ulf R.</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Rapp, Ulf R.</dc:creator> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


My Account