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Peroxynitrite provides the peroxide tone for PGHS-2-dependent prostacyclin synthesis in vascular smooth muscle cells

Peroxynitrite provides the peroxide tone for PGHS-2-dependent prostacyclin synthesis in vascular smooth muscle cells

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SCHILDKNECHT, Stefan, Markus BACHSCHMID, Volker ULLRICH, 2005. Peroxynitrite provides the peroxide tone for PGHS-2-dependent prostacyclin synthesis in vascular smooth muscle cells. In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 19(9), pp. 1169-1171. ISSN 0892-6638. eISSN 1530-6860

@article{Schildknecht2005-07Perox-38695, title={Peroxynitrite provides the peroxide tone for PGHS-2-dependent prostacyclin synthesis in vascular smooth muscle cells}, year={2005}, doi={10.1096/fj.04-3465fje}, number={9}, volume={19}, issn={0892-6638}, journal={The FASEB journal : official publication of the Federation of American Societies for Experimental Biology}, pages={1169--1171}, author={Schildknecht, Stefan and Bachschmid, Markus and Ullrich, Volker} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38695"> <dc:language>eng</dc:language> <dc:contributor>Bachschmid, Markus</dc:contributor> <dc:creator>Schildknecht, Stefan</dc:creator> <dc:contributor>Ullrich, Volker</dc:contributor> <dcterms:abstract xml:lang="eng">Endotoxin-treated vascular smooth muscle cells (VSMCs) were recently shown to release high amounts of prostacyclin (PGI2) dependent on the induction of prostaglandin endoperoxide synthase-2 (PGHS-2). In contrast to endothelial PGI2-synthase, for which nitration and inhibition by peroxynitrite was reported, addition of SIN-1 as a peroxynitrite-generating system did not cause inhibition but rather doubled PGI2 release by VSMC. The hypothesis of peroxynitrite supplementing an unsaturated peroxide tone for PGHS-2 was supported by H2O2 exerting the same effect. Studies performed with purified PGHS-2 revealed maximal elevation of enzyme activity in the presence of equimolar concentrations of *NO and *O2-, which together form peroxynitrite, while excessive production of either one radical was inhibitory. Most importantly, 6-keto-PGF1alpha formation by intact VSMC depended on a nearly equimolar generation of *NO and *O2- for providing the endogenous peroxide tone. These findings, together with the observation that an excess of exogenously added *NO, as well as uric acid as a scavenger of peroxynitrite potently reduced PGI2 release, underlined the role of peroxynitrite as the dominating and rate-limiting intracellular mediator of peroxide tone in VSMC. The results allow us to postulate a new cross-talk between the *NO and the prostanoid pathways with a crucial role for peroxynitrite in providing the peroxide tone for a continuous activation of PGHS-2.</dcterms:abstract> <dcterms:issued>2005-07</dcterms:issued> <dcterms:title>Peroxynitrite provides the peroxide tone for PGHS-2-dependent prostacyclin synthesis in vascular smooth muscle cells</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-03T09:14:50Z</dcterms:available> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38695"/> <dc:creator>Bachschmid, Markus</dc:creator> <dc:contributor>Schildknecht, Stefan</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-03T09:14:50Z</dc:date> <dc:creator>Ullrich, Volker</dc:creator> </rdf:Description> </rdf:RDF>

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