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Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model

Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model

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NEUHAUS, Klaus, Marina C. LAMPARTER, Benjamin ZÖLCH, Richard LANDSTORFER, Svenja SIMON, Britta SPANIER, Matthias A. EHRMANN, Rudi F. VOGEL, 2017. Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model. In: Archives of Microbiology. 199(2), pp. 203-213. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-016-1291-8

@article{Neuhaus2017Probi-38638, title={Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model}, year={2017}, doi={10.1007/s00203-016-1291-8}, number={2}, volume={199}, issn={0302-8933}, journal={Archives of Microbiology}, pages={203--213}, author={Neuhaus, Klaus and Lamparter, Marina C. and Zölch, Benjamin and Landstorfer, Richard and Simon, Svenja and Spanier, Britta and Ehrmann, Matthias A. and Vogel, Rudi F.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38638"> <dc:contributor>Simon, Svenja</dc:contributor> <dc:creator>Lamparter, Marina C.</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38638"/> <dc:creator>Simon, Svenja</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dc:language>eng</dc:language> <dcterms:issued>2017</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-27T08:55:38Z</dc:date> <dc:contributor>Vogel, Rudi F.</dc:contributor> <dc:contributor>Ehrmann, Matthias A.</dc:contributor> <dc:creator>Landstorfer, Richard</dc:creator> <dcterms:title>Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model</dcterms:title> <dc:creator>Ehrmann, Matthias A.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Spanier, Britta</dc:creator> <dc:contributor>Lamparter, Marina C.</dc:contributor> <dc:contributor>Zölch, Benjamin</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-27T08:55:38Z</dcterms:available> <dc:contributor>Spanier, Britta</dc:contributor> <dc:contributor>Landstorfer, Richard</dc:contributor> <dc:creator>Vogel, Rudi F.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Neuhaus, Klaus</dc:contributor> <dc:creator>Zölch, Benjamin</dc:creator> <dcterms:abstract xml:lang="eng">Enterohemorrhagic E. coli O157:H7 (EHEC) shorten the lifespan of Caenorhabditis elegans compared to avirulent bacteria. Co-feeding EHEC with Enterococcus faecalis Symbioflor(®) significantly increased the worms' lifespan. The transcriptome of EHEC grown in vitro with or without Symbioflor(®) was analyzed using RNA-seq. The analysis revealed downregulation of several virulence-associated genes in the presence of Symbioflor(®), including virulence key genes (e.g., LEE, flagellum, quorum-sensing). The downregulation of the LEE genes was corroborated by lux-transposon mutants. Upregulated genes included acid response genes, due to a decrease in pH exerted by Symbioflor(®). Further genes indicate cellular stress in EHEC (e.g. prophage/mobile elements involved in excision, cell lysis, and cell division inhibition). Thus, the observed protection of C. elegans during an EHEC infection by the probiotic Symbioflor(®) is suggested to be caused by triggering concomitant transcriptomic changes. To verify the biological relevance of this modulation, exemplary genes found to be influenced by Symbioflor(®) were knocked out (fliD, espB, Z3136, Z3917, and L7052). The lifespan of nematodes changed when using knock-outs as food source and the effect could be complemented in trans. In summary, Symbioflor(®) appears to be a protective probiotic in the nematode model.</dcterms:abstract> <dc:creator>Neuhaus, Klaus</dc:creator> </rdf:Description> </rdf:RDF>

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