Type of Publication: | Journal article |
Publication status: | Published |
Author: | Neuhaus, Klaus; Lamparter, Marina C.; Zölch, Benjamin; Landstorfer, Richard; Simon, Svenja; Spanier, Britta; Ehrmann, Matthias A.; Vogel, Rudi F. |
Year of publication: | 2017 |
Published in: | Archives of Microbiology ; 199 (2017), 2. - pp. 203-213. - ISSN 0302-8933. - eISSN 1432-072X |
Pubmed ID: | 27655246 |
DOI (citable link): | https://dx.doi.org/10.1007/s00203-016-1291-8 |
Summary: |
Enterohemorrhagic E. coli O157:H7 (EHEC) shorten the lifespan of Caenorhabditis elegans compared to avirulent bacteria. Co-feeding EHEC with Enterococcus faecalis Symbioflor(®) significantly increased the worms' lifespan. The transcriptome of EHEC grown in vitro with or without Symbioflor(®) was analyzed using RNA-seq. The analysis revealed downregulation of several virulence-associated genes in the presence of Symbioflor(®), including virulence key genes (e.g., LEE, flagellum, quorum-sensing). The downregulation of the LEE genes was corroborated by lux-transposon mutants. Upregulated genes included acid response genes, due to a decrease in pH exerted by Symbioflor(®). Further genes indicate cellular stress in EHEC (e.g. prophage/mobile elements involved in excision, cell lysis, and cell division inhibition). Thus, the observed protection of C. elegans during an EHEC infection by the probiotic Symbioflor(®) is suggested to be caused by triggering concomitant transcriptomic changes. To verify the biological relevance of this modulation, exemplary genes found to be influenced by Symbioflor(®) were knocked out (fliD, espB, Z3136, Z3917, and L7052). The lifespan of nematodes changed when using knock-outs as food source and the effect could be complemented in trans. In summary, Symbioflor(®) appears to be a protective probiotic in the nematode model.
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Subject (DDC): | 004 Computer Science |
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NEUHAUS, Klaus, Marina C. LAMPARTER, Benjamin ZÖLCH, Richard LANDSTORFER, Svenja SIMON, Britta SPANIER, Matthias A. EHRMANN, Rudi F. VOGEL, 2017. Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model. In: Archives of Microbiology. 199(2), pp. 203-213. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-016-1291-8
@article{Neuhaus2017Probi-38638, title={Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model}, year={2017}, doi={10.1007/s00203-016-1291-8}, number={2}, volume={199}, issn={0302-8933}, journal={Archives of Microbiology}, pages={203--213}, author={Neuhaus, Klaus and Lamparter, Marina C. and Zölch, Benjamin and Landstorfer, Richard and Simon, Svenja and Spanier, Britta and Ehrmann, Matthias A. and Vogel, Rudi F.} }
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