Type of Publication: | Journal article |
Publication status: | Published |
Author: | Schildknecht, Stefan; Bachschmid, Markus; Weber, Klaus; Maass, Dierk; Ullrich, Volker |
Year of publication: | 2005 |
Published in: | Biochemical and biophysical research communications ; 327 (2005), 1. - pp. 43-48. - ISSN 0006-291X. - eISSN 1090-2104 |
Pubmed ID: | 15629427 |
DOI (citable link): | https://dx.doi.org/10.1016/j.bbrc.2004.11.132 |
Summary: |
Lipopolysaccharide (LPS) exposure to cells and tissues can mimic the biochemical events leading to septic shock. Previous data demonstrated a massive upregulation of prostaglandin endoperoxide H2 synthase (PGHS-2), but not NO synthase-2 (NOS-2) in bovine smooth muscle cells (SMC) between 2 and 12 h of LPS exposure. This caused an abundant release of prostacyclin (PGI2) by constitutive PGI2-synthase as a counterregulation to a dysfunctional endothelium. We here report that human as well as bovine SMC mainly respond by the induction of PGHS-2 and the subsequent release of PGI2, whereas rat SMC exhibited a distinct induction of NOS-2 and released significantly higher amounts of *NO compared with cattle and human. The induction of either PGHS-2 or NOS-2 in the three different species investigated seems to be mutually exclusive in the time window of 2-24 h. This finding should be considered in the setup of experimental models for the investigation of septic shock.
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Subject (DDC): | 570 Biosciences, Biology |
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SCHILDKNECHT, Stefan, Markus BACHSCHMID, Klaus WEBER, Dierk MAASS, Volker ULLRICH, 2005. Endotoxin elicits nitric oxide release in rat but prostacyclin synthesis in human and bovine vascular smooth muscle cells. In: Biochemical and biophysical research communications. 327(1), pp. 43-48. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1016/j.bbrc.2004.11.132
@article{Schildknecht2005-02-04Endot-38359, title={Endotoxin elicits nitric oxide release in rat but prostacyclin synthesis in human and bovine vascular smooth muscle cells}, year={2005}, doi={10.1016/j.bbrc.2004.11.132}, number={1}, volume={327}, issn={0006-291X}, journal={Biochemical and biophysical research communications}, pages={43--48}, author={Schildknecht, Stefan and Bachschmid, Markus and Weber, Klaus and Maass, Dierk and Ullrich, Volker} }
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