Activation-dependent modulation of B lymphocyte migration to chemokines

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BRANDES, Marlene, Daniel F. LEGLER, Bernhard SPOERRI, Patrick SCHAERLI, Bernhard MOSER, 2000. Activation-dependent modulation of B lymphocyte migration to chemokines. In: International immunology. 12(9), pp. 1285-1292. ISSN 0953-8178. eISSN 1460-2377

@article{Brandes2000Activ-36726, title={Activation-dependent modulation of B lymphocyte migration to chemokines}, year={2000}, doi={10.1093/intimm/12.9.1285}, number={9}, volume={12}, issn={0953-8178}, journal={International immunology}, pages={1285--1292}, author={Brandes, Marlene and Legler, Daniel F. and Spoerri, Bernhard and Schaerli, Patrick and Moser, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/36726"> <dc:contributor>Brandes, Marlene</dc:contributor> <dc:creator>Brandes, Marlene</dc:creator> <dc:contributor>Spoerri, Bernhard</dc:contributor> <dc:creator>Schaerli, Patrick</dc:creator> <dc:creator>Spoerri, Bernhard</dc:creator> <dc:creator>Moser, Bernhard</dc:creator> <dcterms:title>Activation-dependent modulation of B lymphocyte migration to chemokines</dcterms:title> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/36726"/> <dcterms:abstract xml:lang="eng">In this study we have examined the migration responses of human peripheral blood or tonsillar B lymphocytes to a selection of 27 chemokines. Freshly isolated (CD19(+)) B lymphocytes show greatly impaired in vitro chemotaxis which is overcome by overnight culture. The best responses of cultured B lymphocytes were observed with BCA-1, SLC, ELC and SDF-1, reaching 19-26% of total input cells that have migrated, followed by LARC and TECK with 5-10% of migrated cells, whereas no other chemokine was found to be active. Stimulation of B lymphocytes with lipopolysaccharide or anti-CD40 plus IL-4 resulted in marked enhancement of the migration response to BCA-1, SLC, ELC and SDF-1, reaching 30-60% migrated cells at 12 or 36 h of culture respectively. The activation-dependent increase in the migration efficacy was transient and declined to base level responses after 72 h of culture. Under no circumstances did we detect B lymphocyte chemotaxis to inflammatory chemokines. Also, mobilization of intracellular calcium ([Ca(2+)](i)), an otherwise typical response of leukocytes to chemokines, was not observed. The transient increase in B lymphocyte migration did not correlate with changes in chemokine receptor expression, as evidenced by cell surface staining with antibodies to CXCR4, CXCR5 and CCR6, and by receptor transcript analyses. BCA-1, SLC, ELC and SDF-1 are typical 'housekeeping' chemokines with prominent expression at discrete locations in lymphoid tissues. Modulation of migration to these chemokines may be a critical mechanism for the proper positioning of B lymphocytes during humoral responses in secondary lymphoid tissues.</dcterms:abstract> <dc:creator>Legler, Daniel F.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-17T10:25:56Z</dc:date> <dc:contributor>Schaerli, Patrick</dc:contributor> <dc:contributor>Legler, Daniel F.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-17T10:25:56Z</dcterms:available> <dc:language>eng</dc:language> <dc:contributor>Moser, Bernhard</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dcterms:issued>2000</dcterms:issued> </rdf:Description> </rdf:RDF>

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