Activation-dependent modulation of B lymphocyte migration to chemokines


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BRANDES, Marlene, Daniel F. LEGLER, Bernhard SPOERRI, Patrick SCHAERLI, Bernhard MOSER, 2000. Activation-dependent modulation of B lymphocyte migration to chemokines. In: International immunology. 12(9), pp. 1285-1292. ISSN 0953-8178. eISSN 1460-2377. Available under: doi: 10.1093/intimm/12.9.1285

@article{Brandes2000Activ-36726, title={Activation-dependent modulation of B lymphocyte migration to chemokines}, year={2000}, doi={10.1093/intimm/12.9.1285}, number={9}, volume={12}, issn={0953-8178}, journal={International immunology}, pages={1285--1292}, author={Brandes, Marlene and Legler, Daniel F. and Spoerri, Bernhard and Schaerli, Patrick and Moser, Bernhard} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Brandes, Marlene</dc:contributor> <dcterms:hasPart rdf:resource=""/> <dc:creator>Brandes, Marlene</dc:creator> <dc:contributor>Spoerri, Bernhard</dc:contributor> <dc:creator>Schaerli, Patrick</dc:creator> <dc:creator>Spoerri, Bernhard</dc:creator> <dc:creator>Moser, Bernhard</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:title>Activation-dependent modulation of B lymphocyte migration to chemokines</dcterms:title> <dspace:hasBitstream rdf:resource=""/> <dc:rights>terms-of-use</dc:rights> <bibo:uri rdf:resource=""/> <dc:creator>Legler, Daniel F.</dc:creator> <dcterms:abstract xml:lang="eng">In this study we have examined the migration responses of human peripheral blood or tonsillar B lymphocytes to a selection of 27 chemokines. Freshly isolated (CD19(+)) B lymphocytes show greatly impaired in vitro chemotaxis which is overcome by overnight culture. The best responses of cultured B lymphocytes were observed with BCA-1, SLC, ELC and SDF-1, reaching 19-26% of total input cells that have migrated, followed by LARC and TECK with 5-10% of migrated cells, whereas no other chemokine was found to be active. Stimulation of B lymphocytes with lipopolysaccharide or anti-CD40 plus IL-4 resulted in marked enhancement of the migration response to BCA-1, SLC, ELC and SDF-1, reaching 30-60% migrated cells at 12 or 36 h of culture respectively. The activation-dependent increase in the migration efficacy was transient and declined to base level responses after 72 h of culture. Under no circumstances did we detect B lymphocyte chemotaxis to inflammatory chemokines. Also, mobilization of intracellular calcium ([Ca(2+)](i)), an otherwise typical response of leukocytes to chemokines, was not observed. The transient increase in B lymphocyte migration did not correlate with changes in chemokine receptor expression, as evidenced by cell surface staining with antibodies to CXCR4, CXCR5 and CCR6, and by receptor transcript analyses. BCA-1, SLC, ELC and SDF-1 are typical 'housekeeping' chemokines with prominent expression at discrete locations in lymphoid tissues. Modulation of migration to these chemokines may be a critical mechanism for the proper positioning of B lymphocytes during humoral responses in secondary lymphoid tissues.</dcterms:abstract> <dspace:isPartOfCollection rdf:resource=""/> <dc:date rdf:datatype="">2017-01-17T10:25:56Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Schaerli, Patrick</dc:contributor> <dc:contributor>Legler, Daniel F.</dc:contributor> <dcterms:isPartOf rdf:resource=""/> <dcterms:available rdf:datatype="">2017-01-17T10:25:56Z</dcterms:available> <dcterms:rights rdf:resource=""/> <dc:contributor>Moser, Bernhard</dc:contributor> <dc:language>eng</dc:language> <dcterms:issued>2000</dcterms:issued> </rdf:Description> </rdf:RDF>

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