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Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering

Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering

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SPÄTE, Anne-Katrin, Jeremias DOLD, Ellen BATROFF, Verena SCHART, Daniel E. WIELAND, Oliver BAUDENDISTEL, Valentin WITTMANN, 2016. Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering. In: ChemBioChem. 17(14), pp. 1374-1383. ISSN 1439-4227. eISSN 1439-7633

@article{Spate2016Explo-36656, title={Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering}, year={2016}, doi={10.1002/cbic.201600197}, number={14}, volume={17}, issn={1439-4227}, journal={ChemBioChem}, pages={1374--1383}, author={Späte, Anne-Katrin and Dold, Jeremias and Batroff, Ellen and Schart, Verena and Wieland, Daniel E. and Baudendistel, Oliver and Wittmann, Valentin} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/36656"> <dc:creator>Baudendistel, Oliver</dc:creator> <dcterms:abstract xml:lang="eng">Metabolic glycoengineering (MGE) allows the introduction of unnaturally modified carbohydrates into cellular glycans and their visualization through bioorthogonal ligation. Alkenes, for example, have been used as reporters that can react through inverse-electron-demand Diels-Alder cycloaddition with tetrazines. Earlier, norbornenes were shown to be suitable dienophiles; however, they had not previously been applied for MGE. We synthesized two norbornene-modified mannosamine derivatives that differ in the stereochemistry at the norbornene (exo/endo linkage). Kinetic investigations revealed that the exo derivative reacts more than twice as rapidly as the endo derivative. Through derivatization with 1,2-diamino-4,5-methylenedioxybenzene (DMB) we confirmed that both derivatives are accepted by cells and incorporated after conversion to a sialic acid. In further MGE experiments the incorporated sugars were ligated to a fluorophore and visualized through confocal fluorescence microscopy and flow cytometry.</dcterms:abstract> <dc:creator>Schart, Verena</dc:creator> <dc:creator>Dold, Jeremias</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-13T14:02:23Z</dc:date> <dc:creator>Späte, Anne-Katrin</dc:creator> <dc:contributor>Batroff, Ellen</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-13T14:02:23Z</dcterms:available> <dc:language>eng</dc:language> <dc:creator>Wieland, Daniel E.</dc:creator> <dc:contributor>Dold, Jeremias</dc:contributor> <dc:contributor>Späte, Anne-Katrin</dc:contributor> <dc:creator>Batroff, Ellen</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Baudendistel, Oliver</dc:contributor> <dc:creator>Wittmann, Valentin</dc:creator> <dcterms:title>Exploring the Potential of Norbornene-Modified Mannosamine Derivatives for Metabolic Glycoengineering</dcterms:title> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/36656"/> <dc:contributor>Wittmann, Valentin</dc:contributor> <dc:contributor>Wieland, Daniel E.</dc:contributor> <dc:contributor>Schart, Verena</dc:contributor> <dcterms:issued>2016</dcterms:issued> </rdf:Description> </rdf:RDF>

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