KOPS - Das Institutionelle Repositorium der Universität Konstanz

Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction

Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:76ef478e1913758880feb39faf4e0a52

HANEBUTH, Marie A., Roman KITYK, Sandra J. FRIES, Alok JAIN, Allison KRIEL, Veronique ALBANESE, Tancred FRICKEY, Christine PETER, Matthias P. MAYER, Elke DEUERLING, 2016. Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction. In: Nature Communications. 7, 13695. eISSN 2041-1723

@article{Hanebuth2016-12-05Multi-36508, title={Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction}, year={2016}, doi={10.1038/ncomms13695}, volume={7}, journal={Nature Communications}, author={Hanebuth, Marie A. and Kityk, Roman and Fries, Sandra J. and Jain, Alok and Kriel, Allison and Albanese, Veronique and Frickey, Tancred and Peter, Christine and Mayer, Matthias P. and Deuerling, Elke}, note={Article Number: 13695} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/36508"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-05T09:07:50Z</dc:date> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:creator>Albanese, Veronique</dc:creator> <dc:contributor>Fries, Sandra J.</dc:contributor> <dc:contributor>Albanese, Veronique</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/36508"/> <dc:creator>Kriel, Allison</dc:creator> <dc:creator>Kityk, Roman</dc:creator> <dc:creator>Hanebuth, Marie A.</dc:creator> <dc:contributor>Kriel, Allison</dc:contributor> <dc:contributor>Mayer, Matthias P.</dc:contributor> <dc:creator>Fries, Sandra J.</dc:creator> <dc:creator>Mayer, Matthias P.</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Frickey, Tancred</dc:contributor> <dc:creator>Frickey, Tancred</dc:creator> <dc:contributor>Peter, Christine</dc:contributor> <dc:creator>Deuerling, Elke</dc:creator> <dc:contributor>Kityk, Roman</dc:contributor> <dc:creator>Peter, Christine</dc:creator> <dcterms:issued>2016-12-05</dcterms:issued> <dcterms:abstract xml:lang="eng">Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-05T09:07:50Z</dcterms:available> <dcterms:title>Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction</dcterms:title> <dc:contributor>Hanebuth, Marie A.</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Jain, Alok</dc:creator> <dc:contributor>Jain, Alok</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 05.01.2017 (Informationen über die Zugriffsstatistik)

Hanebuth_0-383854.pdf 83

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto