Bioorthogonally Functionalized NAD+ Analogues for In-Cell Visualization of Poly(ADP-Ribose) Formation

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Angewandte Chemie International Edition. 2016, 55(27), pp. 7660-7664. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201600464
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Poly(ADP-ribos)ylation (PARylation) is a major posttranslational modification and signaling event in most eukaryotes. Fundamental processes like DNA repair and transcription are coordinated by this transient polymer and its binding to proteins. ADP-ribosyltransferases (ARTs) build complex ADP-ribose chains from NAD+ onto various acceptor proteins. Molecular studies of PARylation thus remain challenging. Herein, we present the development of bioorthogonally functionalized NAD+ analogues for the imaging of PARylation in vitro and in cells. Our results show that 2-modified NAD+ analogues perform remarkably well and can be applied to the in-cell visualization of PARylation simultaneously in two colors. This tool gives insight into the substrate scope of ARTs and will help to further elucidate the biological role of PARylation by offering fast optical, multichannel read-outs.

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ISO 690WALLRODT, Sarah, Annette BUNTZ, Yan WANG, Andreas ZUMBUSCH, Andreas MARX, 2016. Bioorthogonally Functionalized NAD+ Analogues for In-Cell Visualization of Poly(ADP-Ribose) Formation. In: Angewandte Chemie International Edition. 2016, 55(27), pp. 7660-7664. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201600464
BibTex
@article{Wallrodt2016Bioor-35848,
  year={2016},
  doi={10.1002/anie.201600464},
  title={Bioorthogonally Functionalized NAD<sup>+</sup> Analogues for In-Cell Visualization of Poly(ADP-Ribose) Formation},
  number={27},
  volume={55},
  issn={0570-0833},
  journal={Angewandte Chemie International Edition},
  pages={7660--7664},
  author={Wallrodt, Sarah and Buntz, Annette and Wang, Yan and Zumbusch, Andreas and Marx, Andreas}
}
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