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Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

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POMOWSKI, Anja, Walter G. ZUMFT, Peter M. H. KRONECK, Oliver EINSLE, 2010. Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri. In: Acta Crystallographica / Section F Structural Biology and Crystallization Communications. 66(11), pp. 1541-1543. ISSN 1744-3091. eISSN 2053-230X. Available under: doi: 10.1107/S1744309110038832

@article{Pomowski2010-11-01Cryst-35807, title={Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri}, year={2010}, doi={10.1107/S1744309110038832}, number={11}, volume={66}, issn={1744-3091}, journal={Acta Crystallographica / Section F Structural Biology and Crystallization Communications}, pages={1541--1543}, author={Pomowski, Anja and Zumft, Walter G. and Kroneck, Peter M. H. and Einsle, Oliver} }

2010-11-01 Nitrous oxide reductase (N<sub>2</sub>OR) from Pseudomonas stutzeri catalyzes the final step in denitrification: the two-electron reduction of nitrous oxide to molecular dinitrogen. Crystals of the enzyme were grown under strict exclusion of dioxygen by sitting-drop vapour diffusion using 2R,3R-butanediol as a cryoprotectant. N<sub>2</sub>OR crystallized in either space group P1 or P6<sub>5</sub>. Interestingly, the key determinant for the resulting space group was the crystallization temperature. Crystals belonging to space group P1 contained four 130 kDa dimers in the asymmetric unit, while crystals belonging to space group P6<sub>5</sub> contained a single dimer in the asymmetric unit. Diffraction data were collected to resolutions better than 2 Å. eng Zumft, Walter G. Kroneck, Peter M. H. 2016-11-02T10:29:02Z Kroneck, Peter M. H. Pomowski, Anja Einsle, Oliver Pomowski, Anja Einsle, Oliver Zumft, Walter G. 2016-11-02T10:29:02Z Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

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