Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können derzeit keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted currently.)
Type of Publication: | Journal article |
Publication status: | Published |
Author: | Fallah, Mohammad A.; Stanglmair, Christoph; Pacholski, Claudia; Hauser, Karin |
Year of publication: | 2016 |
Published in: | Langmuir ; 32 (2016), 29. - pp. 7356-7364. - ISSN 0743-7463. - eISSN 1520-5827 |
Pubmed ID: | 27389421 |
DOI (citable link): | https://dx.doi.org/10.1021/acs.langmuir.6b01742 |
Summary: |
Surface-enhanced infrared absorption spectroscopy (SEIRA) is applied to study protein conformational changes. In general, the appropriate functionalization of metal surfaces with biomolecules remains a challenge if the conformation and activity of the biomolecule shall be preserved. Here we present a SEIRA study to monitor pH-induced conformational changes of poly-l-lysine (PLL) covalently bound to a thin gold layer via self-assembled monolayers (SAMs). We demonstrate that the composition of the SAM is crucial. A SAM of 11-mercaptoundecanonic acid (MUA) can link PLL to the gold layer, but pH-driven conformational transitions were hindered compared to poly-l-lysine in solution. To address this problem, we devised a variety of SAMs, i.e., mixed SAMs of MUA with either octanethiol (OT) or 11-mercapto-1-undecanol (MUoL) and furthermore SAMs of MT(PEG)4 and NHS-PEG10k-SH. These mixed SAMs modify the surface properties by changing the polarity and the morphology of the surface present to nearby PLL molecules. Our experiments reveal that mixed SAMs of MUA-MUoL and SAMs of NHS-PEG10k-SH-MT(PEG)4 are suitable to monitor pH-driven conformational changes of immobilized PLL. These SAMs might be applicable for chemoselective protein immobilization in general.
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Subject (DDC): | 540 Chemistry |
Bibliography of Konstanz: | Yes |
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FALLAH, Mohammad A., Christoph STANGLMAIR, Claudia PACHOLSKI, Karin HAUSER, 2016. Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes. In: Langmuir. 32(29), pp. 7356-7364. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/acs.langmuir.6b01742
@article{Fallah2016-07-26Devis-35675, title={Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes}, year={2016}, doi={10.1021/acs.langmuir.6b01742}, number={29}, volume={32}, issn={0743-7463}, journal={Langmuir}, pages={7356--7364}, author={Fallah, Mohammad A. and Stanglmair, Christoph and Pacholski, Claudia and Hauser, Karin} }