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Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes

Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes

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FALLAH, Mohammad A., Christoph STANGLMAIR, Claudia PACHOLSKI, Karin HAUSER, 2016. Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes. In: Langmuir. 32(29), pp. 7356-7364. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/acs.langmuir.6b01742

@article{Fallah2016-07-26Devis-35675, title={Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes}, year={2016}, doi={10.1021/acs.langmuir.6b01742}, number={29}, volume={32}, issn={0743-7463}, journal={Langmuir}, pages={7356--7364}, author={Fallah, Mohammad A. and Stanglmair, Christoph and Pacholski, Claudia and Hauser, Karin} }

2016-10-18T09:43:58Z eng Stanglmair, Christoph Pacholski, Claudia Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-l-lysine Conformational Changes Pacholski, Claudia Hauser, Karin Fallah, Mohammad A. 2016-07-26 Surface-enhanced infrared absorption spectroscopy (SEIRA) is applied to study protein conformational changes. In general, the appropriate functionalization of metal surfaces with biomolecules remains a challenge if the conformation and activity of the biomolecule shall be preserved. Here we present a SEIRA study to monitor pH-induced conformational changes of poly-l-lysine (PLL) covalently bound to a thin gold layer via self-assembled monolayers (SAMs). We demonstrate that the composition of the SAM is crucial. A SAM of 11-mercaptoundecanonic acid (MUA) can link PLL to the gold layer, but pH-driven conformational transitions were hindered compared to poly-l-lysine in solution. To address this problem, we devised a variety of SAMs, i.e., mixed SAMs of MUA with either octanethiol (OT) or 11-mercapto-1-undecanol (MUoL) and furthermore SAMs of MT(PEG)<sub>4</sub> and NHS-PEG<sub>10</sub>k-SH. These mixed SAMs modify the surface properties by changing the polarity and the morphology of the surface present to nearby PLL molecules. Our experiments reveal that mixed SAMs of MUA-MUoL and SAMs of NHS-PEG<sub>10</sub>k-SH-MT(PEG)<sub>4</sub> are suitable to monitor pH-driven conformational changes of immobilized PLL. These SAMs might be applicable for chemoselective protein immobilization in general. Stanglmair, Christoph 2016-10-18T09:43:58Z Fallah, Mohammad A. Hauser, Karin

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