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The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7

The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7

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WILLIMANN, Katharina, Daniel F. LEGLER, Marcel LOETSCHER, Regula STUBER ROOS, Maria Belen DELGADO, Ian CLARK-LEWIS, Marco BAGGIOLINI, Bernhard MOSER, 1998. <i>The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7</i>. In: European Journal of Immunology. <b>28</b>(6), pp. 2025-2034. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/(SICI)1521-4141(199806)28:06<2025::AID-IMMU2025>3.0.CO;2-C

@article{Willimann1998-06chemo-35558, title={The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7}, year={1998}, doi={10.1002/(SICI)1521-4141(199806)28:06<2025::AID-IMMU2025>3.0.CO;2-C}, number={6}, volume={28}, issn={0014-2980}, journal={European Journal of Immunology}, pages={2025--2034}, author={Willimann, Katharina and Legler, Daniel F. and Loetscher, Marcel and Stuber Roos, Regula and Delgado, Maria Belen and Clark-Lewis, Ian and Baggiolini, Marco and Moser, Bernhard} }

Stuber Roos, Regula The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7 Clark-Lewis, Ian Legler, Daniel F. Secondary lymphoid-tissue chemokine, SLC, also known as exodus-2 and 6Ckine, is a novel CC chemokine with selectivity for T lymphocytes and preferential expression in lymphoid tissues. We have studied its production, receptor usage and biological activities. High levels of SLC mRNA were detected in lymph nodes, the gastrointestinal tract and several gland tissues, but no expression was found by Northern blot analysis in freshly isolated or stimulated blood monocytes and lymphocytes, or neutrophils and eosinophils. In situ hybridization revealed constitutive expression of SLC in the T cell areas and the marginal zone of follicles in lymph nodes and the mucosa-associated lymphoid tissue, but not in B cell areas or sinuses. Comparison with immunocytochemical staining showed similarity between the in situ expression of SLC and the distribution of interdigitating dendritic cells but not with sinus-lining dendritic cells, macrophages or T lymphocytes. SLC induced chemotaxis of T lymphocytes and its activity increased considerably when the cells were conditioned with IL-2 or phytohemagglutinin (PHA). Under optimal conditions SLC had unusually high efficacy and induced the migration of up to 50 % of input T lymphocytes. SLC also induced Ca<sup>2+</sup> mobilization in these cells. Similar responses were obtained with EBI1 ligand chemokine (ELC), and sequential stimulation with both chemokines led to cross-desensitization, suggesting that SLC acts via the ELC receptor, CCR7. This was confirmed using murine pre-B cells stably transfected with CCR7 which bound SLC with high affinity and showed chemotaxis and Ca<sup>2+</sup> mobilization in response to both SLC and ELC. In T lymphocytes PHA and IL-2, which enhanced chemotactic responsiveness, also markedly enhanced CCR7 expression. In contrast to all known chemokine receptors, up-regulation of CCR7 by IL-2 was transient. A maximum was reached in 2-3 days and expression returned to initial levels within 8-10 days. The present study shows that SLC is constitutively produced within the T cell areas of secondary lymphoid organs and attracts T lymphocytes via CCR7. Willimann, Katharina eng Delgado, Maria Belen Willimann, Katharina Delgado, Maria Belen Loetscher, Marcel 2016-10-13T09:49:58Z Loetscher, Marcel Baggiolini, Marco Moser, Bernhard Stuber Roos, Regula 2016-10-13T09:49:58Z Baggiolini, Marco Moser, Bernhard Legler, Daniel F. Clark-Lewis, Ian 1998-06

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