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Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa

Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa


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Prüfsumme: MD5:76917ef678b783f1b726d9135c481708

MUENZNER, Petra, Arnaud KENGMO TCHOUPA, Benedikt KLAUSER, Thomas BRUNNER, Johannes PUTZE, Ulrich DOBRINDT, Christof R. HAUCK, 2016. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa. In: PLOS Pathogens. 12(5), e1005608. ISSN 1553-7366. eISSN 1553-7374

@article{Muenzner2016-05-12Uropa-35094, title={Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa}, year={2016}, doi={10.1371/journal.ppat.1005608}, number={5}, volume={12}, issn={1553-7366}, journal={PLOS Pathogens}, author={Muenzner, Petra and Kengmo Tchoupa, Arnaud and Klauser, Benedikt and Brunner, Thomas and Putze, Johannes and Dobrindt, Ulrich and Hauck, Christof R.}, note={Article Number: e1005608} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/35094"> <dcterms:title>Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa</dcterms:title> <dc:creator>Brunner, Thomas</dc:creator> <dc:contributor>Dobrindt, Ulrich</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/35094"/> <dc:language>eng</dc:language> <dcterms:issued>2016-05-12</dcterms:issued> <dc:creator>Hauck, Christof R.</dc:creator> <dc:creator>Putze, Johannes</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Putze, Johannes</dc:contributor> <dc:creator>Muenzner, Petra</dc:creator> <dc:contributor>Klauser, Benedikt</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-09-02T08:22:42Z</dcterms:available> <dcterms:abstract xml:lang="eng">Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA) family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa) proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa.</dcterms:abstract> <dc:contributor>Kengmo Tchoupa, Arnaud</dc:contributor> <dc:creator>Kengmo Tchoupa, Arnaud</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-09-02T08:22:42Z</dc:date> <dc:contributor>Muenzner, Petra</dc:contributor> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:contributor>Hauck, Christof R.</dc:contributor> <dc:creator>Klauser, Benedikt</dc:creator> <dc:creator>Dobrindt, Ulrich</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 02.09.2016 (Informationen über die Zugriffsstatistik)

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