@article{Qadota2016domai-34900,
  year={2016},
  doi={10.1091/mbc.E15-09-0675},
  title={The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle},
  number={10},
  volume={27},
  issn={1044-2030},
  journal={Molecular Biology of the Cell},
  pages={1606--1620},
  author={Qadota, Hiroshi and Mayans, Olga and Matsunaga, Yohei and McMurry, Jonathan L. and Wilson, Kristy J. and Kwon, Grace E. and Stanford, Rachel and Deehan, Kevin and Tinley, Tina L. and Benian, Guy M.}
}

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    <dc:creator>Deehan, Kevin</dc:creator>
    <dcterms:title>The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle</dcterms:title>
    <dc:contributor>Mayans, Olga</dc:contributor>
    <dcterms:abstract xml:lang="eng">UNC-89 is a giant polypeptide located at the sarcomeric M-line of Caenorhabditis elegans muscle. The human homologue is obscurin. To understand how UNC-89 is localized and functions, we have been identifying its binding partners. Screening a yeast two-hybrid library revealed that UNC-89 interacts with paramyosin. Paramyosin is an invertebrate-specific coiled-coil dimer protein that is homologous to the rod portion of myosin heavy chains and resides in thick filament cores. Minimally, this interaction requires UNC-89's SH3 domain and residues 294-376 of paramyosin and has a KD of ∼1.1 μM. In unc-89 loss-of-function mutants that lack the SH3 domain, paramyosin is found in accumulations. When the SH3 domain is overexpressed, paramyosin is mislocalized. SH3 domains usually interact with a proline-rich consensus sequence, but the region of paramyosin that interacts with UNC-89's SH3 is α-helical and lacks prolines. Homology modeling of UNC-89's SH3 suggests structural features that might be responsible for this interaction. The SH3-binding region of paramyosin contains a "skip residue," which is likely to locally unwind the coiled-coil and perhaps contributes to the binding specificity.</dcterms:abstract>
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    <dc:creator>Qadota, Hiroshi</dc:creator>
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    <dc:creator>Stanford, Rachel</dc:creator>
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    <dc:creator>Wilson, Kristy J.</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-07-28T12:36:50Z</dc:date>
    <dc:contributor>Deehan, Kevin</dc:contributor>
    <dc:creator>McMurry, Jonathan L.</dc:creator>
    <dc:contributor>Wilson, Kristy J.</dc:contributor>
    <dc:creator>Kwon, Grace E.</dc:creator>
    <dc:creator>Mayans, Olga</dc:creator>
    <dc:contributor>Tinley, Tina L.</dc:contributor>
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    <dc:contributor>Benian, Guy M.</dc:contributor>
    <dc:contributor>Qadota, Hiroshi</dc:contributor>
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    <dc:creator>Benian, Guy M.</dc:creator>
    <dc:contributor>Matsunaga, Yohei</dc:contributor>
    <dc:contributor>McMurry, Jonathan L.</dc:contributor>
    <dc:creator>Matsunaga, Yohei</dc:creator>
    <dcterms:issued>2016</dcterms:issued>
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