KOPS - Das Institutionelle Repositorium der Universität Konstanz

An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry

An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

IURAŞCU, Marius-Ionuţ, Osiris MARROQUIN BELAUNZANAR, Claudia COZMA, Ulf PETRAUSCH, Christoph RENNER, Michael PRZYBYLSKI, 2016. An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry. In: Journal of The American Society for Mass Spectrometry. 27(6), pp. 1105-1112. ISSN 1044-0305. eISSN 1879-1123

@article{Iurascu2016HLA-B-34898, title={An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry}, year={2016}, doi={10.1007/s13361-016-1361-9}, number={6}, volume={27}, issn={1044-0305}, journal={Journal of The American Society for Mass Spectrometry}, pages={1105--1112}, author={Iuraşcu, Marius-Ionuţ and Marroquin Belaunzanar, Osiris and Cozma, Claudia and Petrausch, Ulf and Renner, Christoph and Przybylski, Michael} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/34898"> <dc:creator>Petrausch, Ulf</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-07-28T12:28:25Z</dcterms:available> <dcterms:abstract xml:lang="eng">HLA-B27 homodimer formation is believed to be a hallmark of HLA-B27 associated spondyloarthritides. Recently, we have generated a homodimer-specific monoclonal antibody (HD6) and have demonstrated that HLA-B27 homodimer complexes are present on monocytes of healthy HLA-B27 gene carriers at low levels, with significantly increased levels at active disease. The capability of the HD6 antibody to discriminate between correctly formed HLA-B27 heterotrimers and pathology-associated homodimers is striking and cannot be explained by the primary structure of HLA-B27. We hypothesized that HD6 accesses a unique epitope and used affinity-mass spectrometry for its identification. The HD6 antibody was immobilized on an activated sepharose affinity column, and HLA-B27 homodimer characterized for affinity. The epitope was identified by proteolytic epitope excision and MALDI mass spectrometry, and shown to comprise a discontinuous Cys-203- 257-Cys mixed-disulfide peptide structure that is not accessible in HLA-B27 heterotrimers due to protection by noncovalently linked β2-microglobulin. The epitope peptides were synthesized by solid phase peptide synthesis, and the two monomeric peptide components, HLA-B27(203-219) and HLA-B27(257-273), as well as the homo- and hetero-dimeric disulfide linked combinations prepared. The affinity binding constants KD towards the antibodies were determined using a surface acoustic wave (SAW) biosensor, and showed the highest affinity with a KD of approximately 40 nM to the HD6 antibody for the (203-219)-SS-(257-273) mixed disulfide epitope. Graphical Abstract ᅟ.</dcterms:abstract> <dc:creator>Iuraşcu, Marius-Ionuţ</dc:creator> <dcterms:title>An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry</dcterms:title> <dc:creator>Renner, Christoph</dc:creator> <dcterms:issued>2016</dcterms:issued> <dc:creator>Cozma, Claudia</dc:creator> <dc:creator>Marroquin Belaunzanar, Osiris</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-07-28T12:28:25Z</dc:date> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/34898"/> <dc:contributor>Iuraşcu, Marius-Ionuţ</dc:contributor> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:contributor>Petrausch, Ulf</dc:contributor> <dc:contributor>Cozma, Claudia</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Przybylski, Michael</dc:creator> <dc:contributor>Marroquin Belaunzanar, Osiris</dc:contributor> <dc:contributor>Renner, Christoph</dc:contributor> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto