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Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase

Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase

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ASCHENBRENNER, Joos, Andreas MARX, 2016. Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase. In: Nucleic Acids Research. 44(8), pp. 3495-3502. ISSN 0301-5610. eISSN 1362-4962

@article{Aschenbrenner2016-05-05Direc-34853, title={Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase}, year={2016}, doi={10.1093/nar/gkw200}, number={8}, volume={44}, issn={0301-5610}, journal={Nucleic Acids Research}, pages={3495--3502}, author={Aschenbrenner, Joos and Marx, Andreas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/34853"> <dc:contributor>Marx, Andreas</dc:contributor> <dc:language>eng</dc:language> <dcterms:title>Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase</dcterms:title> <dc:creator>Aschenbrenner, Joos</dc:creator> <dc:creator>Marx, Andreas</dc:creator> <dc:contributor>Aschenbrenner, Joos</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-07-21T13:10:21Z</dcterms:available> <dcterms:issued>2016-05-05</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/34853"/> <dcterms:abstract xml:lang="eng">Methylation of the 2'-hydroxyl-group of ribonucleotides is found in all major classes of RNA in eukaryotes and is one of the most abundant posttranscriptional modifications of stable RNAs. In spite of intense studies, the multiple functions of RNA 2'-O-methylation are still not understood. One major obstacle in the field are the technical demanding detection methods, which are typically laborious and do not always deliver unambiguous results. We present a thermostable KlenTaq DNA polymerase variant with significant reverse transcription activity that is able to discriminate 2'-O-methylated from unmethylated RNAs. The engineered enzyme catalyzes DNA synthesis from DNA as well as RNA templates and enables expeditious quantification of 2'-O-methylation of individual nucleotides directly from total RNA extracts by a simple qRT-PCR.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-07-21T13:10:21Z</dc:date> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 21.07.2016 (Informationen über die Zugriffsstatistik)

Aschenbrenner_0-343405.pdf 39

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