KOPS - Das Institutionelle Repositorium der Universität Konstanz

CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide

CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

BURIN, Sandra Mara, Sandro GHISLA, Amanda Tomie OUCHIDA, Alexandre Ferro AISSA, Maria Gabriela Berzoti COELHO, Tássia Rafaella COSTA, Ana Paula Zambuzi Cardoso MARSOLA, Belinda PINTO-SIMÕES, Lusânia Maria Greggi ANTUNES, Fabíola Attié DE CASTRO, 2016. CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide. In: International Journal of Biological Macromolecules. 86, pp. 309-320. ISSN 0141-8130. eISSN 1879-0003. Available under: doi: 10.1016/j.ijbiomac.2016.01.069

@article{Burin2016CRLAA-34275, title={CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide}, year={2016}, doi={10.1016/j.ijbiomac.2016.01.069}, volume={86}, issn={0141-8130}, journal={International Journal of Biological Macromolecules}, pages={309--320}, author={Burin, Sandra Mara and Ghisla, Sandro and Ouchida, Amanda Tomie and Aissa, Alexandre Ferro and Coelho, Maria Gabriela Berzoti and Costa, Tássia Rafaella and Marsola, Ana Paula Zambuzi Cardoso and Pinto-Simões, Belinda and Antunes, Lusânia Maria Greggi and de Castro, Fabíola Attié} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/34275"> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:contributor>Pinto-Simões, Belinda</dc:contributor> <dc:creator>Coelho, Maria Gabriela Berzoti</dc:creator> <dc:creator>Costa, Tássia Rafaella</dc:creator> <dc:creator>Marsola, Ana Paula Zambuzi Cardoso</dc:creator> <dc:contributor>Burin, Sandra Mara</dc:contributor> <dc:contributor>de Castro, Fabíola Attié</dc:contributor> <dc:creator>Antunes, Lusânia Maria Greggi</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide</dcterms:title> <dc:creator>Ouchida, Amanda Tomie</dc:creator> <dc:contributor>Ouchida, Amanda Tomie</dc:contributor> <dc:contributor>Coelho, Maria Gabriela Berzoti</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Marsola, Ana Paula Zambuzi Cardoso</dc:contributor> <dc:contributor>Aissa, Alexandre Ferro</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/34275"/> <dc:creator>Pinto-Simões, Belinda</dc:creator> <dc:creator>Ghisla, Sandro</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dc:creator>de Castro, Fabíola Attié</dc:creator> <dc:creator>Aissa, Alexandre Ferro</dc:creator> <dc:creator>Burin, Sandra Mara</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-06-08T08:35:16Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-06-08T08:35:16Z</dcterms:available> <dcterms:abstract xml:lang="eng">Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Bcr-Abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. Tyrosine kinase inhibitors (TKIs) are effectively used to treat CML; however, CML patients in the advanced (CML-AP) and chronic (CML-CP) phases of the disease are usually resistant to TKI therapy. Thus, it is necessary to seek for novel agents to treat CML, such as the enzyme l-amino acid oxidase from Calloselasma rhodostoma (CR-LAAO) snake venom. We examined the antitumor effect of CR-LAAO in Bcr-Abl(+) cell lines and peripheral blood mononuclear cells (PBMC) from healthy subjects and CML patients. CR-LAAO was more cytotoxic towards Bcr-Abl(+) cell lines than towards healthy subjects' PBMC. The H2O2 produced during the enzymatic action of CR-LAAO mediated its cytotoxic effect. The CR-LAAO induced apoptosis in Bcr-Abl(+) cells, as detected by caspases 3, 8, and 9 activation, loss of mitochondrial membrane potential, and DNA damage. CR-LAAO elicited apoptosis in PBMC from CML-CP patients without TKI treatment more strongly than in PBMC from healthy subjects and TKI-treated CML-CP and CML-AP patients. The antitumor effect of CR-LAAO against Bcr-Abl(+) cells makes this toxin a promising candidate to CML therapy.</dcterms:abstract> <dc:contributor>Costa, Tássia Rafaella</dc:contributor> <dcterms:issued>2016</dcterms:issued> <dc:contributor>Antunes, Lusânia Maria Greggi</dc:contributor> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto