KOPS - Das Institutionelle Repositorium der Universität Konstanz

FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential

FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

FARHAN, Hesso, Majlinda KULLOLLI, Fabien ZOULIM, Valentina MILLARTE, Jessica COTTAREL, Romain PARENT, Sharon PITTERI, Si-Nafa SI-AHMED, Sophie CLÉMENT, Marie-Laure PLISSONNIER, 2016. FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential. In: Journal of General Virology. 97(1), pp. 69-81. ISSN 0022-1317. eISSN 1465-2099

@article{Farhan2016hepat-33953, title={FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential}, year={2016}, doi={10.1099/jgv.0.000331}, number={1}, volume={97}, issn={0022-1317}, journal={Journal of General Virology}, pages={69--81}, author={Farhan, Hesso and Kullolli, Majlinda and Zoulim, Fabien and Millarte, Valentina and Cottarel, Jessica and Parent, Romain and Pitteri, Sharon and Si-Ahmed, Si-Nafa and Clément, Sophie and Plissonnier, Marie-Laure} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33953"> <dc:creator>Farhan, Hesso</dc:creator> <dcterms:issued>2016</dcterms:issued> <dc:contributor>Clément, Sophie</dc:contributor> <dc:creator>Kullolli, Majlinda</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33953"/> <dc:creator>Cottarel, Jessica</dc:creator> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-18T07:10:06Z</dc:date> <dcterms:abstract xml:lang="eng">There is growing evidence that virus particles also contain host cell proteins, which provide viruses with certain properties required for entry and release. A proteomic analysis performed on double-gradient-purified hepatitis C virus (HCV) from two highly viraemic patients identified the phosphatidylinositol 3,5-bisphosphate 5-phosphatase FIG4 (KIAA0274) as part of the viral particles. We validated the association using immunoelectron microscopy, immunoprecipitation and neutralization assays in vitro as well as patient-derived virus particles. RNA interference-mediated reduction of FIG4 expression decreased cholesteryl ester (CE) levels along with intra- and extracellular viral infectivity without affecting HCV RNA levels. Likewise, overexpressing FIG4 increased intracellular CE levels as well as intra- and extracellular viral infectivity without affecting viral RNA levels. Triglyceride levels and lipid droplet (LD) parameters remained unaffected. The 3,5-bisphosphate 5-phosphatase active site of FIG4 was found to strongly condition these results. Whilst FIG4 was found to localize to areas corresponding to viral assembly sites, at the immediate vicinity of LDs in calnexin-positive and HCV core-positive regions, no implication of FIG4 in the secretory pathway of the hepatocytes could be found using either FIG4-null mice, in vitro morphometry or functional assays of the ERGIC/Golgi compartments. This indicates that FIG4-dependent modulation of HCV infectivity is unrelated to alterations in the functionality of the secretory pathway. As a result of the documented implication of CE in the composition and infectivity of HCV particles, these results suggest that FIG4 binds to HCV and modulates particle formation in a CE-related manner.</dcterms:abstract> <dc:contributor>Plissonnier, Marie-Laure</dc:contributor> <dc:contributor>Zoulim, Fabien</dc:contributor> <dc:contributor>Kullolli, Majlinda</dc:contributor> <dc:creator>Millarte, Valentina</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-18T07:10:06Z</dcterms:available> <dc:creator>Clément, Sophie</dc:creator> <dc:creator>Pitteri, Sharon</dc:creator> <dc:contributor>Farhan, Hesso</dc:contributor> <dc:creator>Zoulim, Fabien</dc:creator> <dc:contributor>Pitteri, Sharon</dc:contributor> <dc:contributor>Parent, Romain</dc:contributor> <dc:contributor>Si-Ahmed, Si-Nafa</dc:contributor> <dc:contributor>Millarte, Valentina</dc:contributor> <dc:creator>Parent, Romain</dc:creator> <dc:contributor>Cottarel, Jessica</dc:contributor> <dcterms:title>FIG4 is a hepatitis C virus particle-bound protein implicated in virion morphogenesis and infectivity with cholesteryl ester modulation potential</dcterms:title> <dc:creator>Plissonnier, Marie-Laure</dc:creator> <dc:creator>Si-Ahmed, Si-Nafa</dc:creator> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto