New Light-Sensitive Nucleosides for Caged DNA Strand Breaks

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DUSSY, Adrian, Christoph MEYER, Edith QUENNET, Thomas A. BICKLE, Bernd GIESE, Andreas MARX, 2002. <i>New Light-Sensitive Nucleosides for Caged DNA Strand Breaks</i>. In: ChemBioChem. <b>3</b>(1), pp. 54-60. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/1439-7633(20020104)3:1<54::AID-CBIC54>3.0.CO;2-2

@article{Dussy2002-01-04Light-33843, title={New Light-Sensitive Nucleosides for Caged DNA Strand Breaks}, year={2002}, doi={10.1002/1439-7633(20020104)3:1<54::AID-CBIC54>3.0.CO;2-2}, number={1}, volume={3}, issn={1439-4227}, journal={ChemBioChem}, pages={54--60}, author={Dussy, Adrian and Meyer, Christoph and Quennet, Edith and Bickle, Thomas A. and Giese, Bernd and Marx, Andreas} }

2002-01-04 Giese, Bernd Meyer, Christoph Marx, Andreas Phototriggered bond cleavage has found wide application in chemistry as well as in biology. Nevertheless, there are only a few methods available for site-specific photochemical induction of DNA strand scission despite numerous potential applications. In this study we report the development of new photocleavable nucleotides based on the photochemistry of o-nitrobenzyl esters. The light-sensitive moieties were generated through introduction of o-nitrophenyl groups at the 5′C position of the nucleoside sugar backbone. The newly synthesized, modified nucleosides were incorporated in oligonucleotides and are able to build stable DNA duplexes. In such a way modified oligonucleotides can be cleaved site-specifically upon irradiation with >360 nm light with high efficiency. Furthermore, we show that these modifications can be bypassed in DNA synthesis promoted by Thermus aquaticus DNA polymerase. 2016-05-09T13:54:32Z Meyer, Christoph Dussy, Adrian New Light-Sensitive Nucleosides for Caged DNA Strand Breaks 2016-05-09T13:54:32Z Bickle, Thomas A. Quennet, Edith Giese, Bernd Quennet, Edith Dussy, Adrian Marx, Andreas eng Bickle, Thomas A.

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