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Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites

Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites

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HOFFMANN, Eugenia, Katharina STREICHERT, Nicole NISCHAN, Carina SEITZ, Thomas BRUNNER, Sergej SCHWAGERUS, Christian P. R. HACKENBERGER, Marina RUBINI, 2016. Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites. In: Molecular BioSystems. 12(6), pp. 1750-1755. ISSN 1742-206X. eISSN 1742-2051

@article{Hoffmann2016-05-24Stabi-33836, title={Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites}, year={2016}, doi={10.1039/c5mb00857c}, number={6}, volume={12}, issn={1742-206X}, journal={Molecular BioSystems}, pages={1750--1755}, author={Hoffmann, Eugenia and Streichert, Katharina and Nischan, Nicole and Seitz, Carina and Brunner, Thomas and Schwagerus, Sergej and Hackenberger, Christian P. R. and Rubini, Marina} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33836"> <dc:creator>Nischan, Nicole</dc:creator> <dc:creator>Hackenberger, Christian P. R.</dc:creator> <dc:contributor>Seitz, Carina</dc:contributor> <dc:creator>Schwagerus, Sergej</dc:creator> <dc:contributor>Rubini, Marina</dc:contributor> <dc:contributor>Hoffmann, Eugenia</dc:contributor> <dc:creator>Brunner, Thomas</dc:creator> <dc:contributor>Streichert, Katharina</dc:contributor> <dcterms:issued>2016-05-24</dcterms:issued> <dc:creator>Seitz, Carina</dc:creator> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-09T12:44:40Z</dc:date> <dcterms:title>Stabilization of bacterially expressed erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites</dcterms:title> <dc:creator>Rubini, Marina</dc:creator> <dc:contributor>Schwagerus, Sergej</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33836"/> <dc:language>eng</dc:language> <dc:creator>Streichert, Katharina</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-09T12:44:40Z</dcterms:available> <dc:creator>Hoffmann, Eugenia</dc:creator> <dc:contributor>Nischan, Nicole</dc:contributor> <dcterms:abstract xml:lang="eng">The covalent attachment of polyethylene glycol (PEG) to therapeutic proteins can improve their physicochemical properties. In this work we utilized the non-natural amino acid p-azidophenylalanine (pAzF) in combination with the chemoselective Staudinger-phosphite reaction to install branched PEG chains to recombinant unglycosylated erythropoietin (EPO) at each single naturally occurring glycosylation site. PEGylation with two short 750 or 2000 Da PEG units at positions 24, 38, or 83 significantly decreased unspecific aggregation and proteolytic degradation while biological activity in vitro was preserved or even increased in comparison to full-glycosylated EPO. This site-specific bioconjugation approach permits to analyse the impact of PEGylation at single positions. These results represent an important step towards the engineering of site-specifically modified EPO variants from bacterial expression with increased therapeutic efficacy.</dcterms:abstract> <dc:contributor>Hackenberger, Christian P. R.</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 09.05.2016 (Informationen über die Zugriffsstatistik)

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