KOPS - Das Institutionelle Repositorium der Universität Konstanz

Diatom Vacuolar 1,6-β-transglycosylases can Functionally Complement the Respective Yeast Mutants

Diatom Vacuolar 1,6-β-transglycosylases can Functionally Complement the Respective Yeast Mutants

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:cd881f94a094312090dcf0be105298a9

HUANG, Weichao, Carolina RÍO BÁRTULOS, Peter G. KROTH, 2016. Diatom Vacuolar 1,6-β-transglycosylases can Functionally Complement the Respective Yeast Mutants. In: Journal of Eukaryotic Microbiology. 63(4), pp. 536-546. ISSN 0022-3921. eISSN 1550-7408. Available under: doi: 10.1111/jeu.12298

@article{Huang2016-07Diato-33785, title={Diatom Vacuolar 1,6-β-transglycosylases can Functionally Complement the Respective Yeast Mutants}, year={2016}, doi={10.1111/jeu.12298}, number={4}, volume={63}, issn={0022-3921}, journal={Journal of Eukaryotic Microbiology}, pages={536--546}, author={Huang, Weichao and Río Bártulos, Carolina and Kroth, Peter G.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33785"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-03T09:05:36Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Kroth, Peter G.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:abstract xml:lang="eng">Diatoms are unicellular photoautotrophic algae, which can be found in any aquatic habitat. The main storage carbohydrate of diatoms is chrysolaminarin, a non-linear β-glucan, consisting of a linear 1,3-β-chain with 1,6-β-branches, which is stored in cytoplasmic vacuoles. The metabolic pathways of chrysolaminarin synthesis in diatoms are poorly investigated, therefore we studied two potential 1,6-β-transglycosylases (TGS) of the diatom Phaeodactylum tricornutum which are similar to yeast Kre6 proteins and which potentially are involved in the branching of 1,3-β-glucan chains by adding D-glucose as 1,6-side chains. We genetically fused the full-length diatom TGS proteins to GFP and expressed these constructs in P. tricornutum, demonstrating that the enzymes are apparently located in the vacuoles, which indicates that branching of chrysolaminarin may occur in these organelles. Furthermore, we demonstrated the functionality of the diatom enzymes by expressing TGS1 and 2 proteins in yeast which resulted in a partial complementation of growth deficiencies of a transglycosylase-deficient ∆kre6 yeast strain. This article is protected by copyright. All rights reserved.</dcterms:abstract> <dcterms:issued>2016-07</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-03T09:05:36Z</dc:date> <dc:contributor>Huang, Weichao</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Kroth, Peter G.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33785/1/Huang_0-327113.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33785/1/Huang_0-327113.pdf"/> <dcterms:title>Diatom Vacuolar 1,6-β-transglycosylases can Functionally Complement the Respective Yeast Mutants</dcterms:title> <dc:creator>Huang, Weichao</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Río Bártulos, Carolina</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33785"/> <dc:creator>Río Bártulos, Carolina</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 03.05.2016 (Informationen über die Zugriffsstatistik)

Huang_0-327113.pdf 26

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto