Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

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2016
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Liu, Shaojun
Luo, Jing
Chai, Jing
Ren, Li
Zhou, Yi
Huang, Feng
Liu, Xiaochuan
Chen, Yubao
Zhang, Ya-Ping
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Proceedings of the National Academy of Sciences ; 113 (2016), 5. - S. 1327-1332. - ISSN 0027-8424. - eISSN 1091-6490
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Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) × common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF1. Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF2 hybrid offspring. The goldfish × common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility.
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ISO 690LIU, Shaojun, Jing LUO, Jing CHAI, Li REN, Yi ZHOU, Feng HUANG, Xiaochuan LIU, Yubao CHEN, Axel MEYER, Ya-Ping ZHANG, 2016. Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross. In: Proceedings of the National Academy of Sciences. 113(5), pp. 1327-1332. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.1512955113
BibTex
@article{Liu2016-02-02Genom-33636,
  year={2016},
  doi={10.1073/pnas.1512955113},
  title={Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross},
  number={5},
  volume={113},
  issn={0027-8424},
  journal={Proceedings of the National Academy of Sciences},
  pages={1327--1332},
  author={Liu, Shaojun and Luo, Jing and Chai, Jing and Ren, Li and Zhou, Yi and Huang, Feng and Liu, Xiaochuan and Chen, Yubao and Meyer, Axel and Zhang, Ya-Ping}
}
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