KOPS - The Institutional Repository of the University of Konstanz

Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

Cite This

Files in this item

Checksum: MD5:ec9ecc8165503af1a465b29335697fb9

VERGA, Daniela, Moritz WELTER, Andreas MARX, 2016. Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides. In: Bioorganic & Medicinal Chemistry Letters. 26(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082

@article{Verga2016Seque-33632, title={Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides}, year={2016}, doi={10.1016/j.bmcl.2015.12.082}, number={3}, volume={26}, issn={0960-894X}, journal={Bioorganic & Medicinal Chemistry Letters}, pages={841--844}, author={Verga, Daniela and Welter, Moritz and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33632"> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33632/1/Verga_0-322822.pdf"/> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Verga, Daniela</dc:contributor> <dc:contributor>Marx, Andreas</dc:contributor> <dc:creator>Welter, Moritz</dc:creator> <dcterms:title>Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-20T08:11:38Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:abstract xml:lang="eng">DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.</dcterms:abstract> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-20T08:11:38Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33632/1/Verga_0-322822.pdf"/> <dc:creator>Verga, Daniela</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33632"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:issued>2016</dcterms:issued> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Welter, Moritz</dc:contributor> <dc:creator>Marx, Andreas</dc:creator> </rdf:Description> </rdf:RDF>

Downloads since Apr 20, 2016 (Information about access statistics)

Verga_0-322822.pdf 121

This item appears in the following Collection(s)

Search KOPS


Browse

My Account