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The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline

The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline

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HUNDT, Matthias, Benjamin SCHREIBER, Reiner ECKMANN, Bjørn Tore LUNESTAD, Hannah WÜNNEMAN, Ralf SCHULZ, 2016. The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline. In: Bulletin of Environmental Contamination and Toxicology. 96(2), pp. 144-149. ISSN 0007-4861. eISSN 1432-0800

@article{Hundt2016Effec-33626, title={The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline}, year={2016}, doi={10.1007/s00128-015-1699-x}, number={2}, volume={96}, issn={0007-4861}, journal={Bulletin of Environmental Contamination and Toxicology}, pages={144--149}, author={Hundt, Matthias and Schreiber, Benjamin and Eckmann, Reiner and Lunestad, Bjørn Tore and Wünneman, Hannah and Schulz, Ralf} }

Schreiber, Benjamin Marking of fish otoliths with oxytetracycline and tetracycline is a widely used method to evaluate the<sup></sup> effectiveness of stocking operations. Available protocols for the labeling of fish specify a number of factors influencing mark quality and potential risk for fish during marking. This study investigates the influence of water hardness on mortality of freshwater fish during marking with OTC. In order to pursue this question complexation of OTC with Mg<sup>2+</sup> and Ca<sup>2+ </sup>cations was measured spectrophotometrically. Furthermore, zebrafish (Danio rerio) were immersed in OTC solutions (1200 mg/L; 48 h immersion) combined with varying levels of water hardness (5.5, 15.5, 25.5, 32.5°dH). The amount of OTC-Mg-Ca-complexes was positively correlated to water hardness. Moreover, it could be demonstrated that mortality of zebrafish during marking varied as a factor of water hardness. Highest mortalities occurred at the lowest (5.5°dH) and the highest (32.5°dH) tested levels during marking with OTC. Hundt, Matthias Hundt, Matthias The Effect of Water Hardness on Mortality of Zebrafish (Danio rerio) During Exposure to Oxytetracycline Lunestad, Bjørn Tore Schulz, Ralf Eckmann, Reiner Schulz, Ralf 2016-04-20T07:27:28Z Schreiber, Benjamin 2016 eng Wünneman, Hannah Wünneman, Hannah Eckmann, Reiner Lunestad, Bjørn Tore 2016-04-20T07:27:28Z

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