KOPS - The Institutional Repository of the University of Konstanz

Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

REHM, Charlotte, Benedikt KLAUSER, Jörg S. HARTIG, 2015. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach. In: PONCHON, Luc, ed.. RNA Scaffolds : Methods and Protocols. New York:Springer, pp. 127-140. ISBN 978-1-4939-2729-6. Available under: doi: 10.1007/978-1-4939-2730-2_11

@incollection{Rehm2015Engin-33211, title={Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach}, year={2015}, doi={10.1007/978-1-4939-2730-2_11}, number={1316}, isbn={978-1-4939-2729-6}, address={New York}, publisher={Springer}, series={Methods in Molecular Biology}, booktitle={RNA Scaffolds : Methods and Protocols}, pages={127--140}, editor={Ponchon, Luc}, author={Rehm, Charlotte and Klauser, Benedikt and Hartig, Jörg S.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33211"> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-03T13:53:15Z</dcterms:available> <dc:contributor>Klauser, Benedikt</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Rehm, Charlotte</dc:contributor> <dcterms:title>Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach</dcterms:title> <dc:contributor>Hartig, Jörg S.</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Klauser, Benedikt</dc:creator> <dcterms:issued>2015</dcterms:issued> <dc:creator>Hartig, Jörg S.</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33211"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Rehm, Charlotte</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-03T13:53:15Z</dc:date> <dcterms:abstract xml:lang="eng">Artificial RNA switches are an emerging class of genetic controllers suitable for synthetic biology applications. Aptazymes are fusions composed of an aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofactors, and most importantly are reprogrammable with respect to ligand selectivity and the RNA function to be regulated. Technologies that enable the generation of aptazymes to defined input ligands are of interest for the construction of biocomputing devices and biosensing applications. In this chapter we present a method that facilitates the in vivo screening of randomized pools of aptazymes in mammalian cells.</dcterms:abstract> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


Browse

My Account