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Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd<sup>3+</sup>-DOTA

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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WOJCIECHOWSKI, Filip, Andreas GROSS, Isabelle T. HOLDER, Laura KNÖRR, Malte DRESCHER, Jörg S. HARTIG, 2015. Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA. In: Chemical communications. 51(72), pp. 13850-13853. ISSN 0009-241X. eISSN 1364-548X. Available under: doi: 10.1039/c5cc04234h

@article{Wojciechowski2015Pulse-33144, title={Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA}, year={2015}, doi={10.1039/c5cc04234h}, number={72}, volume={51}, issn={0009-241X}, journal={Chemical communications}, pages={13850--13853}, author={Wojciechowski, Filip and Groß, Andreas and Holder, Isabelle T. and Knörr, Laura and Drescher, Malte and Hartig, Jörg S.} }

Wojciechowski, Filip Drescher, Malte Knörr, Laura eng Gd<sup>3+</sup> is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd<sup>3+</sup>-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels. 2015 Groß, Andreas 2016-02-29T13:33:55Z Groß, Andreas 2016-02-29T13:33:55Z Holder, Isabelle T. Drescher, Malte terms-of-use Knörr, Laura Holder, Isabelle T. Hartig, Jörg S. Hartig, Jörg S. Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd<sup>3+</sup>-DOTA Wojciechowski, Filip

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