Enzymatic synthesis of peptides on a solid support


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HADDOUB, Rose, Martin DAUNER, Fiona A. STEFANOWICZ, Valeria BARATTINI, Nicolas LAURENT, Sabine L. FLITSCH, 2009. Enzymatic synthesis of peptides on a solid support. In: Organic & Biomolecular Chemistry. 7(4), pp. 665-670. Available under: doi: 10.1039/B816847D

@article{Haddoub2009Enzym-3304, title={Enzymatic synthesis of peptides on a solid support}, year={2009}, doi={10.1039/B816847D}, number={4}, volume={7}, journal={Organic & Biomolecular Chemistry}, pages={665--670}, author={Haddoub, Rose and Dauner, Martin and Stefanowicz, Fiona A. and Barattini, Valeria and Laurent, Nicolas and Flitsch, Sabine L.} }

Publ. in: Organic & Biomolecular Chemistry 7 (2009), 4, pp. 665-670 Flitsch, Sabine L. 2009 Dauner, Martin Haddoub, Rose Laurent, Nicolas Barattini, Valeria Haddoub, Rose Laurent, Nicolas 2011-03-23T10:19:58Z Enzymatic synthesis of peptides on a solid support Flitsch, Sabine L. Barattini, Valeria Stefanowicz, Fiona A. Stefanowicz, Fiona A. Dauner, Martin 2011-03-23T10:19:58Z We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold. terms-of-use eng

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