Toxicity of organic and inorganic mercury species in differentiated human neurons and human astrocytes

Lohren, Hanna; Blagojevic, Lara; Fitkau, Romy; Ebert, Franziska; Schildknecht, Stefan; Leist, Marcel; Schwerdtle, Tanja

Toxicity of organic and inorganic mercury species in differentiated human neurons and human astrocytes

Dateien

Lohren_0-313654.pdfGröße: 983.94 KBDownloads: 1559

Datum

2015

Autor:innen

Lohren, Hanna

Blagojevic, Lara

Fitkau, Romy

Ebert, Franziska

Schildknecht, Stefan

Leist, Marcel

Schwerdtle, Tanja

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Trace Elements in Medicine and Biology. 2015, 32, pp. 200-208. ISSN 0946-672X. eISSN 1878-3252. Available under: doi: 10.1016/j.jtemb.2015.06.008

Zusammenfassung

Organic mercury (Hg) species exert their toxicity primarily in the central nervous system. The food relevant Hg species methylmercury (MeHg) has been frequently studied regarding its neurotoxic effects in vitro and in vivo. Neurotoxicity of thiomersal, which is used as a preservative in medical preparations, is to date less characterised. Due to dealkylation of organic Hg or oxidation of elemental Hg, inorganic Hg is present in the brain albeit these species are not able to readily cross the blood brain barrier. This study compared for the first time toxic effects of organic MeHg chloride (MeHgCl) and thiomersal as well as inorganic mercury chloride (HgCl2) in differentiated human neurons (LUHMES) and human astrocytes (CCF-STTG1). The three Hg species differ in their degree and mechanism of toxicity in those two types of brain cells. Generally, neurons are more susceptible to Hg species induced cytotoxicity as compared to astrocytes. This might be due to the massive cellular mercury uptake in the differentiated neurons. The organic compounds exerted stronger cytotoxic effects as compared to inorganic HgCl2. In contrast to HgCl2 exposure, organic Hg compounds seem to induce the apoptotic cascade in neurons following low-level exposure. No indicators for apoptosis were identified for both inorganic and organic mercury species in astrocytes. Our studies clearly demonstrate species-specific toxic mechanisms. A mixed exposure towards all Hg species in the brain can be assumed. Thus, prospectively coexposure studies as well as cocultures of neurons and astrocytes could provide additional information in the investigation of Hg induced neurotoxicity.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

LOHREN, Hanna, Lara BLAGOJEVIC, Romy FITKAU, Franziska EBERT, Stefan SCHILDKNECHT, Marcel LEIST, Tanja SCHWERDTLE, 2015. Toxicity of organic and inorganic mercury species in differentiated human neurons and human astrocytes. In: Journal of Trace Elements in Medicine and Biology. 2015, 32, pp. 200-208. ISSN 0946-672X. eISSN 1878-3252. Available under: doi: 10.1016/j.jtemb.2015.06.008

BibTex

@article{Lohren2015Toxic-32917,
  year={2015},
  doi={10.1016/j.jtemb.2015.06.008},
  title={Toxicity of organic and inorganic mercury species in differentiated human neurons and human astrocytes},
  volume={32},
  issn={0946-672X},
  journal={Journal of Trace Elements in Medicine and Biology},
  pages={200--208},
  author={Lohren, Hanna and Blagojevic, Lara and Fitkau, Romy and Ebert, Franziska and Schildknecht, Stefan and Leist, Marcel and Schwerdtle, Tanja}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/32917">
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/32917"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/32917/1/Lohren_0-313654.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-02-10T09:10:00Z</dc:date>
    <dc:creator>Ebert, Franziska</dc:creator>
    <dc:creator>Fitkau, Romy</dc:creator>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dc:creator>Blagojevic, Lara</dc:creator>
    <dc:creator>Leist, Marcel</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Lohren, Hanna</dc:creator>
    <dc:contributor>Schwerdtle, Tanja</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Lohren, Hanna</dc:contributor>
    <dcterms:issued>2015</dcterms:issued>
    <dc:language>eng</dc:language>
    <dc:contributor>Ebert, Franziska</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/32917/1/Lohren_0-313654.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-02-10T09:10:00Z</dcterms:available>
    <dc:contributor>Blagojevic, Lara</dc:contributor>
    <dc:contributor>Schildknecht, Stefan</dc:contributor>
    <dc:creator>Schwerdtle, Tanja</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:title>Toxicity of organic and inorganic mercury species in differentiated human neurons and human astrocytes</dcterms:title>
    <dc:creator>Schildknecht, Stefan</dc:creator>
    <dcterms:abstract xml:lang="eng">Organic mercury (Hg) species exert their toxicity primarily in the central nervous system. The food relevant Hg species methylmercury (MeHg) has been frequently studied regarding its neurotoxic effects in vitro and in vivo. Neurotoxicity of thiomersal, which is used as a preservative in medical preparations, is to date less characterised. Due to dealkylation of organic Hg or oxidation of elemental Hg, inorganic Hg is present in the brain albeit these species are not able to readily cross the blood brain barrier. This study compared for the first time toxic effects of organic MeHg chloride (MeHgCl) and thiomersal as well as inorganic mercury chloride (HgCl2) in differentiated human neurons (LUHMES) and human astrocytes (CCF-STTG1). The three Hg species differ in their degree and mechanism of toxicity in those two types of brain cells. Generally, neurons are more susceptible to Hg species induced cytotoxicity as compared to astrocytes. This might be due to the massive cellular mercury uptake in the differentiated neurons. The organic compounds exerted stronger cytotoxic effects as compared to inorganic HgCl2. In contrast to HgCl2 exposure, organic Hg compounds seem to induce the apoptotic cascade in neurons following low-level exposure. No indicators for apoptosis were identified for both inorganic and organic mercury species in astrocytes. Our studies clearly demonstrate species-specific toxic mechanisms. A mixed exposure towards all Hg species in the brain can be assumed. Thus, prospectively coexposure studies as well as cocultures of neurons and astrocytes could provide additional information in the investigation of Hg induced neurotoxicity.</dcterms:abstract>
    <dc:contributor>Fitkau, Romy</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja