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Phytoene Desaturase from Oryza sativa : Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis

Phytoene Desaturase from Oryza sativa : Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis

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GEMMECKER, Sandra, Patrick SCHAUB, Julian KOSCHMIEDER, Anton BRAUSEMANN, Friedel DREPPER, Marta RODRIGUEZ-FRANCO, Sandro GHISLA, Bettina WARSCHEID, Oliver EINSLE, Peter BEYER, 2015. Phytoene Desaturase from Oryza sativa : Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis. In: PLOS ONE. 10(7), e0131717. eISSN 1932-6203

@article{Gemmecker2015Phyto-32857, title={Phytoene Desaturase from Oryza sativa : Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis}, year={2015}, doi={10.1371/journal.pone.0131717}, number={7}, volume={10}, journal={PLOS ONE}, author={Gemmecker, Sandra and Schaub, Patrick and Koschmieder, Julian and Brausemann, Anton and Drepper, Friedel and Rodriguez-Franco, Marta and Ghisla, Sandro and Warscheid, Bettina and Einsle, Oliver and Beyer, Peter}, note={Article Number: e0131717} }

2015 Beyer, Peter Schaub, Patrick Warscheid, Bettina Ghisla, Sandro Koschmieder, Julian Brausemann, Anton Einsle, Oliver Koschmieder, Julian Beyer, Peter Gemmecker, Sandra Schaub, Patrick Brausemann, Anton 2016-02-04T09:02:13Z Einsle, Oliver Drepper, Friedel Recombinant phytoene desaturase (PDS-His<sub>6</sub>) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by molecular oxygen, serve as a final electron acceptor defining PDS as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. The herbicidal PDS-inhibitor norflurazon is capable of arresting the reaction by stabilizing the intermediary FAD<sub>red</sub>, while an excess of the quinone acceptor relieves this blockage, indicating competition. The enzyme requires its homo-oligomeric association for activity. The sum of data collected through gel permeation chromatography, non-denaturing polyacrylamide electrophoresis, chemical cross-linking, mass spectrometry and electron microscopy techniques indicate that the high-order oligomers formed in solution are the basis for an active preparation. Of these, a tetramer consisting of dimers represents the active unit. This is corroborated by our preliminary X-ray structural analysis that also revealed similarities of the protein fold with the sequence-inhomologous bacterial phytoene desaturase CRTI and other oxidoreductases of the GR2-family of flavoproteins. This points to an evolutionary relatedness of CRTI and PDS yielding different carotene desaturation sequences based on homologous protein folds. Drepper, Friedel Warscheid, Bettina Ghisla, Sandro Rodriguez-Franco, Marta Gemmecker, Sandra eng Phytoene Desaturase from Oryza sativa : Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis 2016-02-04T09:02:13Z Rodriguez-Franco, Marta

Dateiabrufe seit 04.02.2016 (Informationen über die Zugriffsstatistik)

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