Publikationstyp: | Zeitschriftenartikel |
Publikationsstatus: | Published |
URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-0-301649 |
Autor/innen: | Rajabi, Khadijeh; Reuther, Julia; Deuerling, Elke; Radford, Sheena E.; Ashcroft, Alison E. |
Erscheinungsjahr: | 2015 |
Erschienen in: | Protein Science ; 24 (2015), 8. - S. 1282-1291. - ISSN 0961-8368. - eISSN 1469-896X |
Pubmed ID: | 25970093 |
DOI (zitierfähiger Link): | https://dx.doi.org/10.1002/pro.2702 |
Zusammenfassung: |
The kinetics and thermodynamics of protein folding are commonly studied in vitro by denaturing/renaturing intact protein sequences. How these folding mechanisms relate to de novo folding that occurs as the nascent polypeptide emerges from the ribosome is much less well understood. Here, we have employed limited proteolysis followed by mass spectrometry analyses to compare directly free and ribosome-tethered polypeptide chains of the Src-homology 3 (SH3) domain and its unfolded variant, SH3-m10. The disordered variant was found to undergo faster proteolysis than SH3. Furthermore, the trypsin cleavage patterns observed show minor, but significant, differences for the free and ribosome-bound nascent chains, with significantly fewer tryptic peptides detected in the presence of ribosome. The results highlight the utility of limited proteolysis coupled with mass spectrometry for the structural analysis of these complex systems, and pave the way for detailed future analyses by combining this technique with chemical labeling methods (for example, hydrogen-deuterium exchange, photochemical oxidation) to analyze protein folding in real time, including in the presence of additional ribosome-associated factors.
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Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
Schlagwörter: | Src-homology 3, circular dichroism spectroscopy, limited proteolysis, nanoelectrospray ionisation-mass spectrometry, ribosome-nascent chain complexes, trypsin digest |
Link zur Lizenz: | Nutzungsbedingungen |
Universitätsbibliographie: | Ja |
RAJABI, Khadijeh, Julia REUTHER, Elke DEUERLING, Sheena E. RADFORD, Alison E. ASHCROFT, 2015. A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry. In: Protein Science. 24(8), pp. 1282-1291. ISSN 0961-8368. eISSN 1469-896X. Available under: doi: 10.1002/pro.2702
@article{Rajabi2015compa-32681, title={A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry}, year={2015}, doi={10.1002/pro.2702}, number={8}, volume={24}, issn={0961-8368}, journal={Protein Science}, pages={1282--1291}, author={Rajabi, Khadijeh and Reuther, Julia and Deuerling, Elke and Radford, Sheena E. and Ashcroft, Alison E.} }
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