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Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor

Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor

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PATIL, Yogita, Madan JUNGHARE, Michael PESTER, Nicolai MÜLLER, Bernhard SCHINK, 2015. Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor. In: International Journal of Systematic and Evolutionary Microbiology. 65(10), pp. 3289-3296. ISSN 1466-5026. eISSN 1466-5034

@article{Patil2015-10-01Anaer-32344, title={Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor}, year={2015}, doi={10.1099/ijsem.0.000410}, number={10}, volume={65}, issn={1466-5026}, journal={International Journal of Systematic and Evolutionary Microbiology}, pages={3289--3296}, author={Patil, Yogita and Junghare, Madan and Pester, Michael and Müller, Nicolai and Schink, Bernhard} }

Pester, Michael eng Pester, Michael Müller, Nicolai Schink, Bernhard 2015-12-04T13:26:08Z A novel strictly anaerobic, mesophilic bacterium was enriched and isolated with gluconate as sole substrate from a methanogenic sludge collected from a biogas reactor. Cells of strain GluBS11<sup>T</sup> stained Gram-positive and were non-motile, straight rods, measuring 3.0-4.5 × 0.8-1.2 μm. The temperature range for growth was 15-37 °C, with optimal growth at 30 °C, the pH range was 6.5-8.5, with optimal growth at pH 7, and the generation time under optimal conditions was 60 min. API Rapid 32A reactions were positive for α-galactosidase, α-glucosidase and β-glucosidase and negative for catalase and oxidase. A broad variety of substrates was utilized, including gluconate, glucose, fructose, maltose, sucrose, lactose, galactose, melezitose, melibiose, mannitol, erythritol, glycerol and aesculin. Products of gluconate fermentation were ethanol, acetate, formate, H<sub>2</sub> and CO<sub>2</sub>. Neither sulfate nor nitrate served as an electron acceptor. Predominant cellular fatty acids (>10 %) were C<sub>14 : 0</sub>, C<sub>16 : 0</sub>, C<sub>16 : 1</sub>ω7c/iso-C<sub>15 : 0</sub> 2-OH and C<sub>18 : 1</sub>ω7c. The DNA G+C content of strain GluBS11<sup>T</sup> was 44.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data revealed that strain GluBS11<sup>T</sup> is a member of subcluster XIVa within the order Clostridiales. The closest cultured relatives are Clostridium herbivorans (93.1 % similarity to the type strain), Clostridium populeti (93.3 %), Eubacterium uniforme (92.4 %) and Clostridium polysaccharolyticum (91.5 %). Based on this 16S rRNA gene sequence divergence (>6.5 %) as well as on chemotaxonomic and phenotypic differences from these taxa, strain GluBS11<sup>T</sup> is considered to represent a novel genus and species, for which the name Anaerobium acetethylicum gen. nov., sp. nov. is proposed. The type strain of Anaerobium acetethylicum is GluBS11<sup>T</sup> ( = LMG 28619<sup>T</sup> = KCTC 15450<sup>T</sup> = DSM 29698<sup>T</sup>). Junghare, Madan Schink, Bernhard Patil, Yogita Müller, Nicolai 2015-12-04T13:26:08Z Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor 2015-10-01 Junghare, Madan Patil, Yogita

Dateiabrufe seit 04.12.2015 (Informationen über die Zugriffsstatistik)

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