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Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator

Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator

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Prüfsumme: MD5:8ba98acb740f68e04e3c7ccbe4a742de

WU, Xun, Jiandong WU, Hongzhao LI, Daniel F. LEGLER, Aaron J. MARSHALL, Francis LIN, 2015. Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator. In: Journal of Immunological Methods. 419, pp. 9-17. ISSN 0022-1759. eISSN 1872-7905

@article{Wu2015Analy-31535, title={Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator}, year={2015}, doi={10.1016/j.jim.2015.02.008}, volume={419}, issn={0022-1759}, journal={Journal of Immunological Methods}, pages={9--17}, author={Wu, Xun and Wu, Jiandong and Li, Hongzhao and Legler, Daniel F. and Marshall, Aaron J. and Lin, Francis} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/31535"> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/31535"/> <dc:contributor>Wu, Xun</dc:contributor> <dc:contributor>Legler, Daniel F.</dc:contributor> <dcterms:abstract xml:lang="eng">T lymphocyte migration is crucial for adaptive immunity. Manipulation of signaling molecules controlling cell migration combined with in-vitro cell migration analysis provides a powerful research approach. Microfluidic devices, which can precisely configure chemoattractant gradients and allow quantitative single cell analysis, have been increasingly applied to cell migration and chemotaxis studies. However, there are a very limited number of published studies involving microfluidic migration analysis of genetically manipulated immune cells. In this study, we describe a simple microfluidic method for quantitative analysis of T cells expressing transfected chemokine receptors and other cell migration signaling probes. Using this method, we demonstrated chemotaxis of Jurkat transfectants expressing wild-type or C-terminus mutated CCR7 within a gradient of chemokine CCL19, and characterized the difference in transfectant migration mediated by wild-type and mutant CCR7. The EGFP-tagged CCR7 allows identification of CCR7-expressing transfectants in cell migration analysis and microscopy assessment of CCR7 dynamics. Collectively, our study demonstrated the effective use of the microfluidic method for studying CCR7 mediated T cell transfectant migration. We envision this developed method will provide a useful platform to functionally test various signaling mechanisms at the cell migration level.</dcterms:abstract> <dc:contributor>Wu, Jiandong</dc:contributor> <dc:contributor>Li, Hongzhao</dc:contributor> <dc:creator>Wu, Xun</dc:creator> <dc:creator>Li, Hongzhao</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Lin, Francis</dc:contributor> <dc:creator>Marshall, Aaron J.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-08-06T10:52:56Z</dc:date> <dc:contributor>Marshall, Aaron J.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-08-06T10:52:56Z</dcterms:available> <dc:creator>Legler, Daniel F.</dc:creator> <dcterms:title>Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator</dcterms:title> <dc:creator>Lin, Francis</dc:creator> <dc:language>eng</dc:language> <dcterms:issued>2015</dcterms:issued> <dc:creator>Wu, Jiandong</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 06.08.2015 (Informationen über die Zugriffsstatistik)

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