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Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula : a pyrosequencing approach

Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula : a pyrosequencing approach

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Prüfsumme: MD5:05cee43d49fae5db3c8be2c50ea66137

KLEINTEICH, Julia, Frank HILDEBRAND, Susie A. WOOD, Samuel CIRÉS, Ramsy AGHA, Antonio QUESADA, David A. PEARCE, Peter CONVEY, Frithjof C. KÜPPER, Daniel R. DIETRICH, 2014. Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula : a pyrosequencing approach. In: Antarctic Science. 26(5), pp. 521-532. ISSN 0954-1020. eISSN 1365-2079. Available under: doi: 10.1017/S0954102014000145

@article{Kleinteich2014Diver-30352, title={Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula : a pyrosequencing approach}, year={2014}, doi={10.1017/S0954102014000145}, number={5}, volume={26}, issn={0954-1020}, journal={Antarctic Science}, pages={521--532}, author={Kleinteich, Julia and Hildebrand, Frank and Wood, Susie A. and Cirés, Samuel and Agha, Ramsy and Quesada, Antonio and Pearce, David A. and Convey, Peter and Küpper, Frithjof C. and Dietrich, Daniel R.} }

Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium (c. 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g<sup>-1</sup> organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g<sup>-1</sup> organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts. Agha, Ramsy Cirés, Samuel eng Wood, Susie A. Convey, Peter Kleinteich, Julia Küpper, Frithjof C. Agha, Ramsy Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula : a pyrosequencing approach Quesada, Antonio Kleinteich, Julia 2015-03-17T14:52:09Z Convey, Peter Dietrich, Daniel R. 2014 Pearce, David A. Hildebrand, Frank Dietrich, Daniel R. Cirés, Samuel Pearce, David A. Küpper, Frithjof C. Hildebrand, Frank 2015-03-17T14:52:09Z Quesada, Antonio Wood, Susie A.

Dateiabrufe seit 17.03.2015 (Informationen über die Zugriffsstatistik)

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