A general design strategy for protein-responsive riboswitches in mammalian cells

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AUSLÄNDER, Simon, Pascal STÜCHELI, Charlotte REHM, David AUSLÄNDER, Jörg S. HARTIG, Martin FUSSENEGGER, 2014. A general design strategy for protein-responsive riboswitches in mammalian cells. In: Nature Methods. 11, pp. 1154-1160. ISSN 1548-7091. eISSN 1548-7105

@article{Auslander2014gener-30093, title={A general design strategy for protein-responsive riboswitches in mammalian cells}, year={2014}, doi={10.1038/nmeth.3136}, volume={11}, issn={1548-7091}, journal={Nature Methods}, pages={1154--1160}, author={Ausländer, Simon and Stücheli, Pascal and Rehm, Charlotte and Ausländer, David and Hartig, Jörg S. and Fussenegger, Martin} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/30093"> <dcterms:issued>2014</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-26T14:33:22Z</dc:date> <dcterms:title>A general design strategy for protein-responsive riboswitches in mammalian cells</dcterms:title> <dc:contributor>Ausländer, Simon</dc:contributor> <dc:contributor>Rehm, Charlotte</dc:contributor> <dc:creator>Rehm, Charlotte</dc:creator> <dcterms:abstract xml:lang="eng">RNAs are ideal for the design of gene switches that can monitor and program cellular behavior because of their high modularity and predictable structure-function relationship. We have assembled an expression platform with an embedded modular ribozyme scaffold that correlates self-cleavage activity of designer ribozymes with transgene translation in bacteria and mammalian cells. A design approach devised to screen ribozyme libraries in bacteria and validate variants with functional tertiary stem-loop structures in mammalian cells resulted in a designer ribozyme with a protein-binding nutR-boxB stem II and a selected matching stem I. In a mammalian expression context, this designer ribozyme exhibited dose-dependent translation control by the N-peptide, had rapid induction kinetics and could be combined with classic small molecule–responsive transcription control modalities to construct complex, programmable genetic circuits.</dcterms:abstract> <dc:creator>Ausländer, David</dc:creator> <dc:contributor>Ausländer, David</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-26T14:33:22Z</dcterms:available> <dc:creator>Fussenegger, Martin</dc:creator> <dc:creator>Hartig, Jörg S.</dc:creator> <dc:contributor>Stücheli, Pascal</dc:contributor> <dc:contributor>Hartig, Jörg S.</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Ausländer, Simon</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/30093"/> <dc:contributor>Fussenegger, Martin</dc:contributor> <dc:creator>Stücheli, Pascal</dc:creator> </rdf:Description> </rdf:RDF>

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