KOPS - Das Institutionelle Repositorium der Universität Konstanz

Catalytic Scope of the Thiamine-Dependent Multifunctional Enzyme Cyclohexane-1,2-dione Hydrolase

Catalytic Scope of the Thiamine-Dependent Multifunctional Enzyme Cyclohexane-1,2-dione Hydrolase

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

LOSCHONSKY, Sabrina, Simon WALTZER, Sonja FRAAS, Tobias WACKER, Susana L. A. ANDRADE, Peter M. H. KRONECK, Michael MÜLLER, 2014. Catalytic Scope of the Thiamine-Dependent Multifunctional Enzyme Cyclohexane-1,2-dione Hydrolase. In: ChemBioChem. 15(3), pp. 389-392. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201300673

@article{Loschonsky2014Catal-30066, title={Catalytic Scope of the Thiamine-Dependent Multifunctional Enzyme Cyclohexane-1,2-dione Hydrolase}, year={2014}, doi={10.1002/cbic.201300673}, number={3}, volume={15}, issn={1439-4227}, journal={ChemBioChem}, pages={389--392}, author={Loschonsky, Sabrina and Waltzer, Simon and Fraas, Sonja and Wacker, Tobias and Andrade, Susana L. A. and Kroneck, Peter M. H. and Müller, Michael} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/30066"> <dc:contributor>Fraas, Sonja</dc:contributor> <dc:contributor>Loschonsky, Sabrina</dc:contributor> <dcterms:title>Catalytic Scope of the Thiamine-Dependent Multifunctional Enzyme Cyclohexane-1,2-dione Hydrolase</dcterms:title> <dcterms:abstract xml:lang="eng">The thiamine diphosphate (ThDP)-dependent enzyme cyclohexane-1,2-dione hydrolase (CDH) was expressed in Escherichia coli and purified by affinity chromatography (Ni-NTA). Recombinant CDH showed the same C-C bond-cleavage and C-C bond-formation activities as the native enzyme. Furthermore, we have shown that CDH catalyzes the asymmetric cross-benzoin reaction of aromatic aldehydes and (decarboxylated) pyruvate (up to quantitative conversion, 92–99 % ee). CDH accepts also hydroxybenzaldehydes and nitrobenzaldehydes; these previously have not (or only in rare cases) been known as substrates of other ThDP-dependent enzymes. On a semipreparative scale, sterically demanding 4-(tert-butyl)benzaldehyde and 2-naphthaldehyde were transformed into the corresponding 2-hydroxy ketone products in high yields. Additionally, certain benzaldehydes with electron withdrawing substituents were identified as potential inhibitors of the ligase activity of CDH.</dcterms:abstract> <dc:creator>Kroneck, Peter M. H.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-25T14:59:49Z</dcterms:available> <dc:creator>Müller, Michael</dc:creator> <dc:creator>Loschonsky, Sabrina</dc:creator> <dc:creator>Waltzer, Simon</dc:creator> <dc:creator>Fraas, Sonja</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Andrade, Susana L. A.</dc:creator> <dc:creator>Wacker, Tobias</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:issued>2014</dcterms:issued> <dc:contributor>Andrade, Susana L. A.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Kroneck, Peter M. H.</dc:contributor> <dc:contributor>Waltzer, Simon</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/30066"/> <dc:contributor>Müller, Michael</dc:contributor> <dc:contributor>Wacker, Tobias</dc:contributor> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-02-25T14:59:49Z</dc:date> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto