Structure of BamA, an essential factor in outer membrane protein biogenesis


Dateien zu dieser Ressource

Prüfsumme: MD5:16e7f05ff1a4a0ac0ff2bfc14eba3d4f

ALBRECHT, Reinhard, Monika SCHÜTZ, Philipp OBERHETTINGER, Michaela FAULSTICH, Ivan BERMEJO, Thomas RUDEL, Kay DIEDERICHS, Kornelius ZETH, 2014. Structure of BamA, an essential factor in outer membrane protein biogenesis. In: Acta Crystallographica Section D : Biological Crystallography. 70(6), pp. 1779-1789. ISSN 0907-4449. eISSN 1399-0047. Available under: doi: 10.1107/S1399004714007482

@article{Albrecht2014Struc-29871, title={Structure of BamA, an essential factor in outer membrane protein biogenesis}, year={2014}, doi={10.1107/S1399004714007482}, number={6}, volume={70}, issn={0907-4449}, journal={Acta Crystallographica Section D : Biological Crystallography}, pages={1779--1789}, author={Albrecht, Reinhard and Schütz, Monika and Oberhettinger, Philipp and Faulstich, Michaela and Bermejo, Ivan and Rudel, Thomas and Diederichs, Kay and Zeth, Kornelius} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:creator>Diederichs, Kay</dc:creator> <bibo:uri rdf:resource=""/> <dc:contributor>Albrecht, Reinhard</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="">2015-02-18T20:08:16Z</dcterms:available> <dspace:isPartOfCollection rdf:resource=""/> <dc:creator>Oberhettinger, Philipp</dc:creator> <dc:language>eng</dc:language> <dcterms:issued>2014</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Structure of BamA, an essential factor in outer membrane protein biogenesis</dcterms:title> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Schütz, Monika</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dcterms:rights rdf:resource=""/> <dc:contributor>Oberhettinger, Philipp</dc:contributor> <dc:contributor>Bermejo, Ivan</dc:contributor> <dcterms:abstract xml:lang="eng">Outer membrane protein (OMP) biogenesis is an essential process for maintaining the bacterial cell envelope and involves the β-barrel assembly machinery (BAM) for OMP recognition, folding and assembly. In Escherichia coli this function is orchestrated by five proteins: the integral outer membrane protein BamA of the Omp85 superfamily and four associated lipoproteins. To unravel the mechanism underlying OMP folding and insertion, the structure of the E. coli BamA β-barrel and P5 domain was determined at 3 Å resolution. These data add information beyond that provided in the recently published crystal structures of BamA from Haemophilus ducreyi and Neisseria gonorrhoeae and are a valuable basis for the interpretation of pertinent functional studies. In an `open' conformation, E. coli BamA displays a significant degree of flexibility between P5 and the barrel domain, which is indicative of a multi-state function in substrate transfer. E. coli BamA is characterized by a discontinuous β-barrel with impaired β1–β16 strand interactions denoted by only two connecting hydrogen bonds and a disordered C-terminus. The 16-stranded barrel surrounds a large cavity which implies a function in OMP substrate binding and partial folding. These findings strongly support a mechanism of OMP biogenesis in which substrates are partially folded inside the barrel cavity and are subsequently released laterally into the lipid bilayer.</dcterms:abstract> <dc:contributor>Zeth, Kornelius</dc:contributor> <dc:creator>Zeth, Kornelius</dc:creator> <dc:contributor>Schütz, Monika</dc:contributor> <dc:creator>Bermejo, Ivan</dc:creator> <dc:contributor>Diederichs, Kay</dc:contributor> <dc:date rdf:datatype="">2015-02-18T20:08:16Z</dc:date> <dc:creator>Faulstich, Michaela</dc:creator> <dcterms:hasPart rdf:resource=""/> <dc:creator>Albrecht, Reinhard</dc:creator> <dspace:hasBitstream rdf:resource=""/> <dc:contributor>Faulstich, Michaela</dc:contributor> <dc:contributor>Rudel, Thomas</dc:contributor> <dc:creator>Rudel, Thomas</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 18.02.2015 (Informationen über die Zugriffsstatistik)

Albrecht_0-263735.pdf 217

Das Dokument erscheint in:

KOPS Suche


Mein Benutzerkonto