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Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na<sup>+</sup>-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae

Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae

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Prüfsumme: MD5:c26026ea43a919bcf63a7bba680c51c7

VOHL, Georg, Ruslan NEDIELKOV, Björn CLAUSSEN, Marco S. CASUTT, Thomas VORBURGER, Kay DIEDERICHS, Heiko M. MÖLLER, Julia STEUBER, Günter FRITZ, 2014. Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae. In: Acta Crystallographica Section F : Structural Biology and Crystallization Communications. 70(7), pp. 987-992. eISSN 1744-3091

@article{Vohl2014Cryst-29824, title={Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae}, year={2014}, doi={10.1107/S2053230X14009881}, number={7}, volume={70}, journal={Acta Crystallographica Section F : Structural Biology and Crystallization Communications}, pages={987--992}, author={Vohl, Georg and Nedielkov, Ruslan and Claussen, Björn and Casutt, Marco S. and Vorburger, Thomas and Diederichs, Kay and Möller, Heiko M. and Steuber, Julia and Fritz, Günter} }

Vohl, Georg Steuber, Julia Casutt, Marco S. 2015-02-11T09:04:03Z 2015-02-11T09:04:03Z Fritz, Günter Fritz, Günter Vorburger, Thomas Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na<sup>+</sup>-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae Vohl, Georg Claussen, Björn Möller, Heiko M. Vorburger, Thomas Nedielkov, Ruslan Casutt, Marco S. Diederichs, Kay Claussen, Björn The Na<sup>+</sup>-translocating NADH:ubiquinone oxidoreductase (Na<sup>+</sup>-NQR) from Vibrio cholerae is a membrane protein complex consisting of six different subunits NqrA-NqrF. The major domains of the NqrA and NqrC subunits were heterologously expressed in Escherichia coli and crystallized. The structure of NqrA<sub>1-377</sub> was solved in space groups C222<sub>1</sub> and P2<sub>1</sub> by SAD phasing and molecular replacement at 1.9 and 2.1 Å resolution, respectively. NqrC devoid of the transmembrane helix was co-expressed with ApbE to insert the flavin mononucleotide group covalently attached to Thr225. The structure was determined by molecular replacement using apo-NqrC of Parabacteroides distasonis as search model at 1.8 Å resolution. Diederichs, Kay Nedielkov, Ruslan Möller, Heiko M. eng Steuber, Julia 2014

Dateiabrufe seit 11.02.2015 (Informationen über die Zugriffsstatistik)

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