KOPS - Das Institutionelle Repositorium der Universität Konstanz

Identification and Affinity-Quantification of ß-Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry

Identification and Affinity-Quantification of ß-Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:d1d5a1635cb3ca6c0c7a3af058a32577

SLAMNOIU, Stefan, Camelia VLAD, Mihaela STUMBAUM, Adrian MOISE, Kathrin LINDNER, Nicole ENGEL, Mar VILANOVA, Mireia DIAZ, Christiaan KARREMAN, Marcel LEIST, Thomas CIOSSEK, Bastian HENGERER, Marta VILASECA, Michael PRZYBYLSKI, 2014. Identification and Affinity-Quantification of ß-Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry. In: Journal of The American Society for Mass Spectrometry. 25(8), pp. 1472-1481. ISSN 1044-0305. eISSN 1879-1123

@article{Slamnoiu2014Ident-29741, title={Identification and Affinity-Quantification of ß-Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry}, year={2014}, doi={10.1007/s13361-014-0904-1}, number={8}, volume={25}, issn={1044-0305}, journal={Journal of The American Society for Mass Spectrometry}, pages={1472--1481}, author={Slamnoiu, Stefan and Vlad, Camelia and Stumbaum, Mihaela and Moise, Adrian and Lindner, Kathrin and Engel, Nicole and Vilanova, Mar and Diaz, Mireia and Karreman, Christiaan and Leist, Marcel and Ciossek, Thomas and Hengerer, Bastian and Vilaseca, Marta and Przybylski, Michael} }

Diaz, Mireia Karreman, Christiaan Leist, Marcel Hengerer, Bastian Vlad, Camelia Stumbaum, Mihaela Ciossek, Thomas Leist, Marcel Vlad, Camelia Karreman, Christiaan Ciossek, Thomas Vilaseca, Marta Lindner, Kathrin Slamnoiu, Stefan Bioaffinity analysis using a variety of biosensors has become an established tool for detection and quantification of biomolecular interactions. Biosensors, however, are generally limited by the lack of chemical structure information of affinity-bound ligands. On-line bioaffinity-mass spectrometry using a surface-acoustic wave biosensor (SAW-MS) is a new combination providing the simultaneous affinity detection, quantification, and mass spectrometric structural characterization of ligands. We describe here an on-line SAW-MS combination for direct identification and affinity determination, using a new interface for MS of the affinity-isolated ligand eluate. Key element of the SAW-MS combination is a microfluidic interface that integrates affinity-isolation on a gold chip, in-situ sample concentration, and desalting with a microcolumn for MS of the ligand eluate from the biosensor. Suitable MS- acquisition software has been developed that provides coupling of the SAW-MS interface to a Bruker Daltonics ion trap-MS, FTICR-MS, and Waters Synapt-QTOF- MS systems. Applications are presented for mass spectrometric identifications and affinity (K<sub>D</sub>) determinations of the neurodegenerative polypeptides, ß-amyloid (Aß), and pathophysiological and physiological synucleins (α- and ß-synucleins), two key polypeptide systems for Alzheimer’s disease and Parkinson’s disease, respectively. Moreover, first in vivo applications of αSyn polypeptides from brain homogenate show the feasibility of on-line affinity-MS to the direct analysis of biological material. These results demonstrate on-line SAW-bioaffinity-MS as a powerful tool for structural and quantitative analysis of biopolymer interactions. Diaz, Mireia Slamnoiu, Stefan Stumbaum, Mihaela Hengerer, Bastian Moise, Adrian Engel, Nicole 2015-02-04T08:53:20Z Przybylski, Michael Przybylski, Michael 2015-02-04T08:53:20Z Identification and Affinity-Quantification of ß-Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry Engel, Nicole Moise, Adrian 2014 Vilaseca, Marta Vilanova, Mar Vilanova, Mar Lindner, Kathrin eng

Dateiabrufe seit 04.02.2015 (Informationen über die Zugriffsstatistik)

Leist_0-261177.pdf 30

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto