hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase

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KREFT, Stefan G., Michael NASSAL, 2003. hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase. In: Journal of Cell Science. 116(4), pp. 605-616. ISSN 0021-9533. eISSN 1477-9137. Available under: doi: 10.1242/​jcs.00261

@article{Kreft2003hRUL1-29530, title={hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase}, year={2003}, doi={10.1242/​jcs.00261}, number={4}, volume={116}, issn={0021-9533}, journal={Journal of Cell Science}, pages={605--616}, author={Kreft, Stefan G. and Nassal, Michael} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/29530"> <dc:language>eng</dc:language> <dc:contributor>Kreft, Stefan G.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-01-16T12:01:32Z</dcterms:available> <dcterms:title>hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase</dcterms:title> <dc:contributor>Nassal, Michael</dc:contributor> <dc:creator>Nassal, Michael</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29530/1/Kreft_0-269583.pdf"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/29530/1/Kreft_0-269583.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Kreft, Stefan G.</dc:creator> <dcterms:issued>2003</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/29530"/> <dcterms:abstract xml:lang="eng">Cellular as well as viral RNAs are usually found complexed with proteins. In an attempt to identify proteins that interact with transcripts of hepatitis B virus (HBV), a DNA virus that replicates through reverse transcription, a partial cDNA was isolated from a human cDNA expression library whose gene product bound to an HBV-derived RNA. Using an overlapping clone from a molecular hybridization screen a full-length cDNA was assembled. It contained a large open reading frame for a 1208 amino-acid protein of 138 kDa identical to the hypothetical product of the KIAA0675 clone. Closely related sequences are present in mouse cDNA libraries but not in the genomes of lower organisms. The protein sequence contained no known RNA-binding domain and, apart from a probable coiled-coil domain, the only significant homology involved a complete RING-H2 motif. This suggested that the protein might be a novel RNA-binding RING-dependent ubiquitin-protein ligase or E3 enzyme. A motif critical for RNA binding was experimentally mapped to a central Lys-rich region. Binding specificity is either broad or the protein has as yet unknown physiological targets; hence, at present, a potential importance for HBV biology remains open. The RING-H2 domain was functional in and essential for self- and trans-ubiquitylation in vitro and for proteasome-mediated turnover of the protein in vivo. We therefore termed it hRUL138 for human RNA-binding ubiquitin ligase of 138 kDa. hRUL138 mRNAs are expressed at low levels in most tissues. GFP-tagged hRUL138 derivatives were found associated with cytoplasmic structures, possibly the ER, but excluded from the nucleus. The combined presence of RNA binding and E3 activity in hRUL138 raises the possibility that both are mechanistically linked.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-01-16T12:01:32Z</dc:date> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 16.01.2015 (Informationen über die Zugriffsstatistik)

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