The strength of the template effect attracting nucleotides to naked DNA


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KERVIO, Eric, Birgit CLAASEN, Ulrich E. STEINER, Clemens RICHERT, 2014. The strength of the template effect attracting nucleotides to naked DNA. In: Nucleic Acids Research. 42(11), pp. 7409-7420. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gku314

@article{Kervio2014stren-29462, title={The strength of the template effect attracting nucleotides to naked DNA}, year={2014}, doi={10.1093/nar/gku314}, number={11}, volume={42}, issn={0305-1048}, journal={Nucleic Acids Research}, pages={7409--7420}, author={Kervio, Eric and Claasen, Birgit and Steiner, Ulrich E. and Richert, Clemens} }

2014-12-19T10:33:54Z The transmission of genetic information relies on Watson-Crick base pairing between nucleoside phosphates and template bases in template–primer complexes. Enzyme-free primer extension is the purest form of the transmission process, without any chaperon-like effect of polymerases. This simple form of copying of sequences is intimately linked to the origin of life and provides new opportunities for reading genetic information. Here, we report the dissociation constants for complexes between (deoxy)nucleotides and template–primer complexes, as determined by nuclear magnetic resonance and the inhibitory effect of unactivated nucleotides on enzyme-free primer extension. Depending on the sequence context, K<sub>d</sub>´s range from 280 mM for thymidine monophosphate binding to a terminal adenine of a hairpin to 2 mM for a deoxyguanosine monophosphate binding in the interior of a sequence with a neighboring strand. Combined with rate constants for the chemical step of extension and hydrolytic inactivation, our quantitative theory explains why some enzyme-free copying reactions are incomplete while others are not. For example, for GMP binding to ribonucleic acid, inhibition is a significant factor in low-yielding reactions, whereas for amino-terminal DNA hydrolysis of monomers is critical. Our results thus provide a quantitative basis for enzyme-free copying terms-of-use Kervio, Eric 2014 Claasen, Birgit Steiner, Ulrich E. Richert, Clemens The strength of the template effect attracting nucleotides to naked DNA 2014-12-19T10:33:54Z Steiner, Ulrich E. Richert, Clemens eng Kervio, Eric Claasen, Birgit

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