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Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins

Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins

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GUTIÉRREZ ACOSTA, Olga B., David SCHLEHECK, Bernhard SCHINK, 2014. Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins. In: BMC Genomics. 15(1), 584. eISSN 1471-2164. Available under: doi: 10.1186/1471-2164-15-584

@article{GutierrezAcosta2014Aceto-28663, title={Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins}, year={2014}, doi={10.1186/1471-2164-15-584}, number={1}, volume={15}, journal={BMC Genomics}, author={Gutiérrez Acosta, Olga B. and Schleheck, David and Schink, Bernhard}, note={Article Number: 584} }

eng Acetone utilization by sulfate-reducing bacteria : draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins BMC Genomics ; 15 (2014). - 584 Background<br />The sulfate-reducing bacterium Desulfococcus biacutus is able to utilize acetone for growth by an inducible degradation pathway that involves a novel activation reaction for acetone with CO as a co-substrate. The mechanism, enzyme(s) and gene(s) involved in this acetone activation reaction are of great interest because they represent a novel and yet undefined type of activation reaction under strictly anoxic conditions.<br /><br />Results<br />In this study, a draft genome sequence of D. biacutus was established. Sequencing, assembly and annotation resulted in 159 contigs with 5,242,029 base pairs and 4773 predicted genes; 4708 were predicted protein-encoding genes, and 3520 of these had a functional prediction. Proteins and genes were identified that are specifically induced during growth with acetone. A thiamine diphosphate-requiring enzyme appeared to be highly induced during growth with acetone and is probably involved in the activation reaction. Moreover, a coenzyme B<sub>12</sub>- dependent enzyme and proteins that are involved in redox reactions were also induced during growth with acetone.<br /><br />Conclusions<br />We present for the first time the genome of a sulfate reducer that is able to grow with acetone. The genome information of this organism represents an important tool for the elucidation of a novel reaction mechanism that is employed by a sulfate reducer in acetone activation. Schleheck, David Schink, Bernhard 2014 Schleheck, David Schink, Bernhard deposit-license 2014-08-05T08:47:44Z Gutiérrez Acosta, Olga B. Gutiérrez Acosta, Olga B. 2014-08-05T08:47:44Z

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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