Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-286222 |
Author: | Juneja, Puneet; Rao, Ashit; Cölfen, Helmut; Diederichs, Kay; Welte, Wolfram |
Year of publication: | 2014 |
Published in: | Acta Crystallographica Section F Structural Biology Communications ; 70 (2014), 2. - pp. 260-262. - eISSN 2053-230X |
Pubmed ID: | 24637770 |
DOI (citable link): | https://dx.doi.org/10.1107/S2053230X14000880 |
Summary: |
Sea urchin spicules have a calcitic mesocrystalline architecture that is closely associated with a matrix of proteins and amorphous minerals. The mechanism underlying spicule formation involves complex processes encompassing spatio-temporally regulated organic–inorganic interactions. C-type lectin domains are present in several spicule matrix proteins in Strongylocentrotus purpuratus, implying their role in spiculogenesis. In this study, the C-type lectin domain of SM50 was overexpressed, purified and crystallized using a vapour-diffusion method. The crystal diffracted to a resolution of 2.85 Å and belonged to space group P212121, with unit-cell parameters a = 100.6, b = 115.4, c = 130.6 Å, α = β = γ = 90°. Assuming 50% solvent content, six chains are expected to be present in the asymmetric unit.
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Subject (DDC): | 540 Chemistry |
Keywords: | C-type lectins, SM50, spiculogenesis, Strongylocentrotus purpuratus |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
JUNEJA, Puneet, Ashit RAO, Helmut CÖLFEN, Kay DIEDERICHS, Wolfram WELTE, 2014. Crystallization and preliminary X-ray analysis of the C-type lectin domain of the spicule matrix protein SM50 from Strongylocentrotus purpuratus. In: Acta Crystallographica Section F Structural Biology Communications. 70(2), pp. 260-262. eISSN 2053-230X. Available under: doi: 10.1107/S2053230X14000880
@article{Juneja2014-02Cryst-28622, title={Crystallization and preliminary X-ray analysis of the C-type lectin domain of the spicule matrix protein SM50 from Strongylocentrotus purpuratus}, year={2014}, doi={10.1107/S2053230X14000880}, number={2}, volume={70}, journal={Acta Crystallographica Section F Structural Biology Communications}, pages={260--262}, author={Juneja, Puneet and Rao, Ashit and Cölfen, Helmut and Diederichs, Kay and Welte, Wolfram} }
Juneja_286222.pdf | 214 |