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A bacterial DNA quadruplex with exceptional K<sup>+</sup> selectivity and unique structural polymorphism

A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism

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Prüfsumme: MD5:03af91f97d3df29bb4c1c286d719ec97

REHM, Charlotte, Isabelle T. HOLDER, Andreas GROSS, Filip WOJCIECHOWSKI, Maximilian URBAN, Malte SINN, Malte DRESCHER, Jörg S. HARTIG, 2014. A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism. In: Chemical Science. 5(7), pp. 2809-2818. ISSN 2041-6520. eISSN 2041-6539. Available under: doi: 10.1039/C4SC00440J

@article{Rehm2014bacte-28430, title={A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism}, year={2014}, doi={10.1039/C4SC00440J}, number={7}, volume={5}, issn={2041-6520}, journal={Chemical Science}, pages={2809--2818}, author={Rehm, Charlotte and Holder, Isabelle T. and Groß, Andreas and Wojciechowski, Filip and Urban, Maximilian and Sinn, Malte and Drescher, Malte and Hartig, Jörg S.} }

Wojciechowski, Filip Groß, Andreas Urban, Maximilian 2014 eng Groß, Andreas The G-rich sequence d[(G<sub>4</sub>CT)<sub>3</sub>G<sub>4</sub>] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum. We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G<sub>4</sub>CT)<sub>3</sub>G<sub>4</sub>] </sub>displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G<sub>4</sub>CT)<sub>3</sub>G<sub>4</sub>] shows a so far unprecedented selectivity for K<sup>+</sup> with even high concentrations of Na<sup>+</sup> unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K<sup>+</sup>-concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion. Chemical Science ; 5 (2014), 7. - S. 2809-2818 deposit-license Holder, Isabelle T. 2014-07-17T07:00:29Z Rehm, Charlotte Hartig, Jörg S. Rehm, Charlotte Sinn, Malte Hartig, Jörg S. Urban, Maximilian A bacterial DNA quadruplex with exceptional K<sup>+</sup> selectivity and unique structural polymorphism Holder, Isabelle T. Sinn, Malte Drescher, Malte Wojciechowski, Filip Drescher, Malte

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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