Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-284300 |
Author: | Rehm, Charlotte; Holder, Isabelle T.; Groß, Andreas; Wojciechowski, Filip; Urban, Maximilian; Sinn, Malte; Drescher, Malte; Hartig, Jörg S. |
Year of publication: | 2014 |
Published in: | Chemical Science ; 5 (2014), 7. - pp. 2809-2818. - ISSN 2041-6520. - eISSN 2041-6539 |
DOI (citable link): | https://dx.doi.org/10.1039/C4SC00440J |
Summary: |
The G-rich sequence d[(G4CT)3G4] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum. We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G4CT)3G4] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G4CT)3G4] shows a so far unprecedented selectivity for K+ with even high concentrations of Na+ unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K+-concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.
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Subject (DDC): | 540 Chemistry |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
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REHM, Charlotte, Isabelle T. HOLDER, Andreas GROSS, Filip WOJCIECHOWSKI, Maximilian URBAN, Malte SINN, Malte DRESCHER, Jörg S. HARTIG, 2014. A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism. In: Chemical Science. 5(7), pp. 2809-2818. ISSN 2041-6520. eISSN 2041-6539. Available under: doi: 10.1039/C4SC00440J
@article{Rehm2014bacte-28430, title={A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism}, year={2014}, doi={10.1039/C4SC00440J}, number={7}, volume={5}, issn={2041-6520}, journal={Chemical Science}, pages={2809--2818}, author={Rehm, Charlotte and Holder, Isabelle T. and Groß, Andreas and Wojciechowski, Filip and Urban, Maximilian and Sinn, Malte and Drescher, Malte and Hartig, Jörg S.} }
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