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Chain-Terminating and Clickable NAD<sup>+</sup> Analogues for Labeling the Target Proteins of ADP-Ribosyltransferases

Chain-Terminating and Clickable NAD+ Analogues for Labeling the Target Proteins of ADP-Ribosyltransferases

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WANG, Yan, Daniel RÖSNER, Magdalena GRZYWA, Andreas MARX, 2014. Chain-Terminating and Clickable NAD+ Analogues for Labeling the Target Proteins of ADP-Ribosyltransferases. In: Angewandte Chemie International Edition. 53(31), pp. 8159-8162. ISSN 1433-7851. eISSN 1521-3773

@article{Wang2014-07-28Chain-28356, title={Chain-Terminating and Clickable NAD+ Analogues for Labeling the Target Proteins of ADP-Ribosyltransferases}, year={2014}, doi={10.1002/anie.201404431}, number={31}, volume={53}, issn={1433-7851}, journal={Angewandte Chemie International Edition}, pages={8159--8162}, author={Wang, Yan and Rösner, Daniel and Grzywa, Magdalena and Marx, Andreas} }

2014-07-14T13:07:56Z Grzywa, Magdalena Marx, Andreas 2014-07-14T13:07:56Z Rösner, Daniel eng Grzywa, Magdalena Rösner, Daniel ADP-ribosyltransferases (ARTs) use NAD<sup>+</sup> as a substrate and play important roles in numerous biological processes, such as the DNA damage response and cell cycle regulation, by transferring multiple ADP-ribose units onto target proteins to form poly(ADP-ribose) (PAR) chains of variable sizes. Efforts to identify direct targets of PARylation, as well as the specific ADP-ribose acceptor sites, must all tackle the complexity of PAR. Herein, we report new NAD<sup>+</sup> analogues that are efficiently processed by wild-type ARTs and lead to chain termination owing to a lack of the required hydroxy group, thereby significantly reducing the complexity of the protein modification. Due to the presence of an alkyne group, these NAD<sup>+</sup> analogues allow subsequent manipulations by click chemistry for labeling with dyes or affinity markers. This study provides insight into the substrate scope of ARTs and might pave the way for the further developments of chemical tools for investigating PAR metabolism. 2014-07-28 Marx, Andreas Chain-Terminating and Clickable NAD<sup>+</sup> Analogues for Labeling the Target Proteins of ADP-Ribosyltransferases Wang, Yan deposit-license Wang, Yan Angewandte Chemie : International Edition ; 53 (2014), 31. - S. 8159-8162

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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