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Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury

Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury

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Prüfsumme: MD5:a653ea8f2e948ebf5a43e50b33c833f0

KÄHLER, Christian M., Jutta WECHSELBERGER, Wolfgang HILBE, Andreas GSCHWENDTNER, Daniela COLLESELLI, Harald NIEDEREGGER, Eva-Maria BONEBERG, Gilbert SPIZZO, Albrecht WENDEL, Eberhard GUNSILIUS, Josef R. PATSCH, Jürg HAMACHER, 2007. Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury. In: Respiratory Research. 8(1), 50. ISSN 1465-9921. eISSN 1465-993X. Available under: doi: 10.1186/1465-9921-8-50

@article{Kahler2007Perip-28140, title={Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury}, year={2007}, doi={10.1186/1465-9921-8-50}, number={1}, volume={8}, issn={1465-9921}, journal={Respiratory Research}, author={Kähler, Christian M. and Wechselberger, Jutta and Hilbe, Wolfgang and Gschwendtner, Andreas and Colleselli, Daniela and Niederegger, Harald and Boneberg, Eva-Maria and Spizzo, Gilbert and Wendel, Albrecht and Gunsilius, Eberhard and Patsch, Josef R. and Hamacher, Jürg}, note={Article Number: 50} }

Wendel, Albrecht Gunsilius, Eberhard Gschwendtner, Andreas Spizzo, Gilbert Hilbe, Wolfgang Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury Colleselli, Daniela Kähler, Christian M. Colleselli, Daniela 2014-06-25T09:11:53Z eng Boneberg, Eva-Maria Niederegger, Harald Gunsilius, Eberhard Gschwendtner, Andreas Respiratory Research ; 8 (2007). - 50 Patsch, Josef R. Wechselberger, Jutta Boneberg, Eva-Maria Patsch, Josef R. Wendel, Albrecht 2007 2014-06-25T09:11:53Z deposit-license Hamacher, Jürg Niederegger, Harald Spizzo, Gilbert Wechselberger, Jutta Background<br /><br /><br />Bone marrow-derived progenitors for both epithelial and endothelial cells have been observed in the lung. Besides mature endothelial cells (EC) that compose the adult vasculature, endothelial progenitor cells (EPC) are supposed to be released from the bone marrow into the peripheral blood after stimulation by distinct inflammatory injuries. Homing of ex vivo generated bone marrow-derived EPC into the injured lung has not been investigated so far. We therefore tested the hypothesis whether homing of EPC in damaged lung tissue occurs after intravenous administration.<br /><br /><br /><br />Methods<br /><br /><br />Ex vivo generated, characterized and cultivated rat bone marrow-derived EPC were investigated for proliferation and vasculogenic properties in vitro. EPC were tested for their homing in a left-sided rat lung transplant model mimicking a severe acute lung injury. EPC were transplanted into the host animal by peripheral administration into the femoral vein (106 cells). Rats were sacrificed 1, 4 or 9 days after lung transplantation and homing of EPC was evaluated by fluorescence microscopy. EPC were tested further for their involvement in vasculogenesis processes occurring in subcutaneously applied Matrigel in transplanted animals.<br /><br /><br /><br />Results<br /><br /><br />We demonstrate the integration of intravenously injected EPC into the tissue of the transplanted left lung suffering from acute lung injury. EPC were localized in vessel walls as well as in destructed lung tissue. Virtually no cells were found in the right lung or in other organs. However, few EPC were found in subcutaneous Matrigel in transplanted rats.<br /><br /><br /><br />Conclusion<br /><br /><br />Transplanted EPC may play an important role in reestablishing the endothelial integrity in vessels after severe injury or at inflamatory sites and might further contribute to vascular repair or wound healing processes in severely damaged tissue. Therapeutic applications of EPC transplantation may ensue. Kähler, Christian M. Hilbe, Wolfgang Hamacher, Jürg

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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