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Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by Genome-wide analysis of single nucleotide polymorphisms : evidence for highly clonal population structure

Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by Genome-wide analysis of single nucleotide polymorphisms : evidence for highly clonal population structure

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Prüfsumme: MD5:6461300920e81b16ebc6ffd720a1ef2a

RATTEI, Thomas, Stephan OTT, Michaela GUTACKER, Jan RUPP, Matthias MAASS, Stefan SCHREIBER, Werner SOLBACH, Thierry WIRTH, Jens GIEFFERS, 2007. Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by Genome-wide analysis of single nucleotide polymorphisms : evidence for highly clonal population structure. In: BMC Genomics. 8(1), 355. ISSN 1471-2164. eISSN 1471-2164

@article{Rattei2007Genet-28138, title={Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by Genome-wide analysis of single nucleotide polymorphisms : evidence for highly clonal population structure}, year={2007}, doi={10.1186/1471-2164-8-355}, number={1}, volume={8}, issn={1471-2164}, journal={BMC Genomics}, author={Rattei, Thomas and Ott, Stephan and Gutacker, Michaela and Rupp, Jan and Maass, Matthias and Schreiber, Stefan and Solbach, Werner and Wirth, Thierry and Gieffers, Jens}, note={Article Number: 355} }

2014-06-24T08:08:33Z Rupp, Jan BMC Genomics ; 8 (2007). - 355 2014-06-24T08:08:33Z Gutacker, Michaela Rattei, Thomas Gieffers, Jens 2007 Gieffers, Jens Rupp, Jan Rattei, Thomas Background<br /><br />Chlamydophila pneumoniae is an obligate intracellular bacterium that replicates in a biphasic life cycle within eukaryotic host cells. Four published genomes revealed an identity of > 99 %. This remarkable finding raised questions about the existence of distinguishable genotypes in correlation with geographical and anatomical origin.<br /><br />Results<br /><br />We studied the genetic diversity of C. pneumoniae by analysing synonymous single nucleotide polymorphisms (sSNPs) that are under reduced selection pressure. We conducted an in silico analysis of the four sequenced genomes, chose 232 representative sSNPs and analysed the loci in 38 C. pneumoniae isolates. We identified 15 different genotypes that were separated in four major clusters. Clusters were not associated with anatomical or geographical origin. However, animal lineages are basal on the C. pneumomiae phylogeny, suggesting a recent transmission to humans through successive bottlenecks some 150,000 years ago. A lack of detectable variation in 17 isolates emphasizes the extraordinary genetic conservation of this species and the high clonality of the population. Moreover, the largest cluster, which encompasses 80% of all analysed strains, is an extremely young clade, that went through an important population expansion some 3,300 years ago.<br /><br />Conclusion<br /><br />sSNPs have proven useful as a sensitive marker to gain new insights into genetic diversity, population structure and evolutionary history of C. pneumoniae. Gutacker, Michaela deposit-license Schreiber, Stefan Maass, Matthias Wirth, Thierry Ott, Stephan Genetic diversity of the obligate intracellular bacterium Chlamydophila pneumoniae by Genome-wide analysis of single nucleotide polymorphisms : evidence for highly clonal population structure Schreiber, Stefan Wirth, Thierry eng Ott, Stephan Maass, Matthias Solbach, Werner Solbach, Werner

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