Composition of agarose substrate affects behavioral output of Drosophila larvae


Dateien zu dieser Ressource

Prüfsumme: MD5:47b9e23af3ffb0a802bc85881c34c202

APOSTOLOPOULOU, Anthi A., Fabian HERSPERGER, Lorena MAZIJA, Annekathrin WIDMANN, Alexander WÜST, Andreas S. THUM, 2014. Composition of agarose substrate affects behavioral output of Drosophila larvae. In: Frontiers in Behavioral Neuroscience. 8, 11. eISSN 1662-5153. Available under: doi: 10.3389/fnbeh.2014.00011

@article{Apostolopoulou2014Compo-27985, title={Composition of agarose substrate affects behavioral output of Drosophila larvae}, year={2014}, doi={10.3389/fnbeh.2014.00011}, volume={8}, journal={Frontiers in Behavioral Neuroscience}, author={Apostolopoulou, Anthi A. and Hersperger, Fabian and Mazija, Lorena and Widmann, Annekathrin and Wüst, Alexander and Thum, Andreas S.}, note={Article Number: 11} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:language>eng</dc:language> <dc:contributor>Widmann, Annekathrin</dc:contributor> <dcterms:rights rdf:resource=""/> <dc:contributor>Apostolopoulou, Anthi A.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Hersperger, Fabian</dc:creator> <dcterms:issued>2014</dcterms:issued> <dc:contributor>Hersperger, Fabian</dc:contributor> <dc:contributor>Thum, Andreas S.</dc:contributor> <dcterms:bibliographicCitation>Frontiers in behavioral neuroscience ; 8 (2014). - 11</dcterms:bibliographicCitation> <dc:creator>Widmann, Annekathrin</dc:creator> <dc:contributor>Wüst, Alexander</dc:contributor> <dc:creator>Thum, Andreas S.</dc:creator> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="">2014-05-30T13:30:15Z</dc:date> <bibo:uri rdf:resource=""/> <dc:creator>Apostolopoulou, Anthi A.</dc:creator> <dc:creator>Mazija, Lorena</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dc:rights>terms-of-use</dc:rights> <dspace:hasBitstream rdf:resource=""/> <dcterms:available rdf:datatype="">2014-05-30T13:30:15Z</dcterms:available> <dcterms:abstract xml:lang="eng">In the last decade the Drosophila larva has evolved into a simple model organism offering the opportunity to integrate molecular genetics with systems neuroscience. This led to a detailed understanding of the neuronal networks for a number of sensory functions and behaviors including olfaction, vision, gustation and learning and memory. Typically, behavioral assays in use exploit simple Petri dish setups with either agarose or agar as a substrate. However, neither the quality nor the concentration of the substrate is generally standardized across these experiments and there is no data available on how larval behavior is affected by such different substrates. Here, we have investigated the effects of different agarose concentrations on several larval behaviors. We demonstrate that agarose concentration is an important parameter, which affects all behaviors tested: preference, feeding, learning and locomotion. Larvae can discriminate between different agarose concentrations, they feed differently on them, they can learn to associate an agarose concentration with an odor stimulus and change locomotion on a substrate of higher agarose concentration. Additionally, we have investigated the effect of agarose concentration on three quinine based behaviors: preference, feeding and learning. We show that in all cases examined the behavioral output changes in an agarose concentration-dependent manner. Our results suggest that comparisons between experiments performed on substrates differing in agarose concentration should be done with caution. It should be taken into consideration that the agarose concentration can affect the behavioral output and thereby the experimental outcomes per se potentially due to the initiation of an escape response or changes in foraging behavior on more rigid substrates.</dcterms:abstract> <dc:creator>Wüst, Alexander</dc:creator> <dc:contributor>Mazija, Lorena</dc:contributor> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:title>Composition of agarose substrate affects behavioral output of Drosophila larvae</dcterms:title> <dcterms:hasPart rdf:resource=""/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Apostolopoulou_279854.pdf 168

Das Dokument erscheint in:

KOPS Suche


Mein Benutzerkonto