Acetone activation by the strictly anaerobic bacterium Desulfococcus biacutus


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GUTIERREZ ACOSTA, Olga Brigida, 2013. Acetone activation by the strictly anaerobic bacterium Desulfococcus biacutus [Dissertation]. Konstanz: University of Konstanz

@phdthesis{GutierrezAcosta2013Aceto-26779, title={Acetone activation by the strictly anaerobic bacterium Desulfococcus biacutus}, year={2013}, author={Gutierrez Acosta, Olga Brigida}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:rights rdf:resource=""/> <dc:creator>Gutierrez Acosta, Olga Brigida</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Acetone activation by the strictly anaerobic bacterium Desulfococcus biacutus</dcterms:title> <dc:rights>terms-of-use</dc:rights> <bibo:uri rdf:resource=""/> <dspace:hasBitstream rdf:resource=""/> <dcterms:issued>2013</dcterms:issued> <dcterms:hasPart rdf:resource=""/> <dspace:isPartOfCollection rdf:resource=""/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:isPartOf rdf:resource=""/> <dcterms:abstract xml:lang="eng">In the context of this dissertation the activation of acetone under strictly anoxic conditions was studied. For that purpose the sulfate-reducing microorganism Desulfococcus biacutus has been used. It is concluded that the activation of acetone by D. biacutus occurs in a novel activating mechanism with CO as cosubstrate. This ATP-dependent carbonylation of acetone leads to the formation of acetoacetaldehyde as intermediate. The sequenced and annotated genome of D. biacutus that is part of this work allowed a better understanding of the activation of acetone, and it opened the doors to further investigations on this topic. For example, with genomic and proteomic analysis we found a TPP-dependent enzyme which might be involved in the activation reaction in the degradation of acetone. Moreover, initial experiments showed that TPP is a cofactor in the initial reaction as it was shown with the use of an ATP analogue. There is also the possibility that a B12 enzyme participates in the acetone degradation pathway. Finally, we conclude that strictly anaerobic activation of ketones and hydrocarbons differs highly from the activation in the presence of oxygen, and that the discovery of this novel acetone activation might help in further investigations of hydrocarbon activation.</dcterms:abstract> <dc:language>eng</dc:language> <dc:contributor>Gutierrez Acosta, Olga Brigida</dc:contributor> <dc:date rdf:datatype="">2014-03-05T08:33:32Z</dc:date> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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